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pRset-rogRFP2

Plasmid

#207875

Purpose

Bacterial expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GW1-rogRFP2

Plasmid

#207876

Purpose

Mammalian expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pWR24

Plasmid

#202771

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR16

Plasmid

#202711

Purpose

integrating bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25 dRING_IRES-mCherry

Plasmid

#198387

Purpose

RNF25 (?RING) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

RING deletion (135-201)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT011-ZF6 1x FLAG-synTF-PPF

Plasmid

#202501

Purpose

Express "PPF" synTF (ZF6)

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

p65-ZF6

Use

Tags

1x FLAG and Palmitoylation-Palmitoylation-Farnesy…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep-D3:Luc

Plasmid

#192990

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D3

Promoter

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDx_mCherry-SYFP2

Plasmid

#189759

Purpose

Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mCherry-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDx_FusionRed-SYFP2

Plasmid

#189760

Purpose

Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

FusionRed-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pVAX1-VSVG-INDIANA2

Plasmid

#196623

Purpose

Express VSV-G protein in mammalian cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

VSV-G

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep-D1:Luc

Plasmid

#192988

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D1

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HyWnt3-ΔRep-D2:Luc

Plasmid

#192989

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D2

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HyWnt3-ΔRep-D4:Luc

Plasmid

#192991

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D4

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_346

Plasmid

#180549

Purpose

Entry vector containing gfasPurple

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

gfasPurple

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA424::tsi5

Plasmid

#192960

Purpose

Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tsi5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::D1141A

Plasmid

#192963

Purpose

Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5_D1141A

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

D1141A

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::9xhis-Tse5

Plasmid

#192962

Purpose

Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep:Luc

Plasmid

#192987

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

Repressor element deleted

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160a

Plasmid

#173756

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160a_sgRNA2/R160a

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU6 &StU6

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160b

Plasmid

#173757

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160b_sgRNA2/160b

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU6 &StU6

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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