Addgene

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pEGFP-rbNHERF1

Plasmid

#31636

Depositor

Edward Weinman

Article

Voltz et al J Biol Chem. 2007 Nov 16. 282(46):33879-

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS

Plasmid

#69220

Purpose

Expresses wild type SpCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTYF-2xGfaABC1D-tTA

Plasmid

#19981

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xGfaABC1D-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only

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pBT374_pattB-pCA-GFP-pA-attBSwa

Plasmid

#52553

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-HA no-pA

Plasmid

#82592

Purpose

3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSuper 44-1

Plasmid

#51597

Purpose

shRNA 1 against rat and mouse Sema4D

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA1

Use

RNAi

Tags

Expression

Mutation

Promoter

U6

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pGHNC5

Plasmid

#24701

Depositor

George Allen

Article

Allen et al Plant Cell. 1996 May . 8(5):899-913.

Insert

Direct Repeats of Tobacco Rb7-6 MARs

Use

E. coli phagemid

Tags

Expression

Mutation

Promoter

Available since

July 8, 2010

Availability

Academic Institutions and Nonprofits only

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pClneoEGFP human RASSF6b

Plasmid

#37021

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

RASSF6b (RASSF6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-H11r1-2-DD-Cas9

Plasmid

#85978

Purpose

Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus cerberus short

Plasmid

#24681

Depositor

Edward De Robertis

Article

Piccolo et al Nature. 1999 Feb 25. 397(6721):707-10.

Insert

cerberus (cer1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Cerberus short

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK8-VHL

Plasmid

#29498

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pGBT-R15

Plasmid

#31826

Depositor

Stephen Ekker

Article

Petzold et al Proc Natl Acad Sci U S A. 2009 Nov 3;106

Insert

Tol2 (t2gtlp4 Zebrafish)

Use

Cre/Lox; Gene trap

Tags

mRFP

Expression

Mutation

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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p3E-YFP-HA no-pA

Plasmid

#82590

Purpose

3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

YFP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

SpCas9n

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBFv-UAS3

Plasmid

#138399

Purpose

10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

UAS

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-Venus

Plasmid

#138388

Purpose

RMCE vector for generating C-terminal Venus fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

Venus

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pPZP FLIPmal-25u

Plasmid

#14476

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPmal

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

W230A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
Wnt5B STOP

Plasmid

#35875

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G0-R20

Plasmid

#101818

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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