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Showing: 8791 - 8820 of 148587 results

  3. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Use
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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  8. pCDNA3 IL-2 signal mFc

    Plasmid
    #128042
    Purpose
    Mammalian expression vector with C terminal IL-2 signal peptide and mFc
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    IL-2 signal peptide and mFc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128042-plasmid-map-sequence-id-254868

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  9. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  13. pCool-mRbx1-His-hCul1-CTP

    Plasmid
    #29519
    Depositor
    Ning Zheng
    Insert
    Rbx1 CUL1 (CUL1, Rbx1 Human, Mouse)
    Use
    Tags
    GST, His (before thrombin), and Thrombin Cut Site…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. pDisplay-mgSrtA

    Plasmid
    #125792
    Purpose
    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
    Depositor
    P. Chen
    Article
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Insert
    mgSrtA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125792-plasmid-map-sequence-id-258287

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  17. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

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  18. pTTP1A

    Plasmid
    #91721
    Purpose
    integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA
    Depositor
    Graham Hatfull
    Article
    Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23
    Type
    Empty backbone
    Use
    Integration-proficient plasmid vectors for mycoba…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    91721-plasmid-map-sequence-id-178356

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  22. QSOX1-bio-His

    Plasmid
    #51825
    Purpose
    Expresses full-length Sulfhydryl oxidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    QSOX1 (QSOX1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51825-plasmid-map-sequence-id-79662

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  28. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Use
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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Showing: 8791 - 8820 of 148587 results