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pcDNA3.1-MS2-HA-HsRoquin1_510-1133_AE

Plasmid

#148613

Purpose

Mammalian Expression of HsRoquin1_510-1133

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1_510-1133 (RC3H1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-GFP beta isoform

Plasmid

#22147

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HSPH1

Plasmid

#19453

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPH1 (HSPH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pOGG012

Plasmid

#113986

Purpose

Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Partition genes (parABCDE)

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inpp5e

Plasmid

#96808

Purpose

cilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-null

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inpp5e

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM D4 BsmBI Myd88 stuffer

Plasmid

#128505

Purpose

pAPM knockdown vector with Myd88 stuffer sequence

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-258 (aka VD1)

Plasmid

#46270

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-258 (aka VD1) (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1-258 (VD1 domain)

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pJZC518

Plasmid

#62277

Purpose

Yeast dCas9 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9

Use

Tags

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K138/139R

Plasmid

#127138

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Tags

Expression

Mutation

K138R/K139R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pDGB3_alpha1

Plasmid

#68228

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTH-PanCen TALE-mVenusN

Plasmid

#191395

Purpose

TALE fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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DCIP

Plasmid

#193860

Purpose

Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

NLS-mCherry-GFP1-10

Use

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-mCherry

Plasmid

#193864

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Use

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-mCherrySTOP

Plasmid

#193866

Purpose

E. coli expression vector for N-ter GFP11 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Use

Tags

6xHis and GFP11

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-851 (Vh)

Plasmid

#46268

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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