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pWR9

Plasmid

#202645

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSD118A

Plasmid

#218140

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0_ZF2-3

Use

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD068F

Plasmid

#218137

Purpose

Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36-eGFP-IRES2-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD057C

Plasmid

#218136

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD113A

Plasmid

#218133

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM189A

Plasmid

#218135

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM154

Plasmid

#218121

Purpose

Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

FKBP12
luxAB

Use

Luciferase

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2 (STOP)

Plasmid

#204204

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Use

Tags

T7x3

Expression

Mammalian

Mutation

Nonsense mutations after Gly1135

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2 (A1134-1135)

Plasmid

#204201

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Use

Tags

T7x3

Expression

Mammalian

Mutation

Gly to Ala 1134 to 1135

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-ER

Plasmid

#214413

Purpose

ER localization of CFAST fused to Halotag-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Use

Tags

CFAST-Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-Halo-ER

Plasmid

#214419

Purpose

ER localization of low-affinity NFAST fused to Halo-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-mApple-mito

Plasmid

#214415

Purpose

Mitochondria localization of low-affinity NFAST fused to mApple-mito

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

mApple-mito

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4(1-147)

Plasmid

#195029

Purpose

The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4 (1-147)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GL4.10 Prg4 promoter-luciferase

Plasmid

#195034

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter (PRG4 Bovine)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-YF

Plasmid

#210723

Purpose

Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR54

Plasmid

#203330

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-DIIG

Plasmid

#201196

Purpose

Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-DIIG

Use

Tags

HisTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-SWG

Plasmid

#201194

Purpose

Expresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-SWG

Use

Tags

HisTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-PEED

Plasmid

#201195

Purpose

Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-PEED

Use

Tags

HisTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox17FNV-3xflag

Plasmid

#206375

Purpose

Expresses 3xflag fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV-3xflag (Sox17 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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