Addgene

We narrowed to 37,276 results for: ina

Showing: 5821 - 5840 of 37276 results

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Use

Tags

DsbC-6His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag3B-ERT2CCre

Plasmid

#106371

Purpose

C-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

ERT2CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBbE5a-Opto-Cre-Vvd

Plasmid

#134404

Purpose

Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB105

Plasmid

#68559

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB130

Plasmid

#68584

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

tdTomato

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB108

Plasmid

#68562

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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p667-UBC-AAK1-V5_IDG-K

Plasmid

#135257

Purpose

Gateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AAK1-V5 (AAK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hMLKL

Plasmid

#208789

Purpose

Inducibly expresses human MLKL, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MLKL (MLKL Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-p54

Plasmid

#35379

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

p54 (NONO Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

A464V

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTD-NStrepHis

Plasmid

#45936

Purpose

use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Use

Tags

HIS-6-tag and Twin-Strep-tag

Expression

Bacterial

Mutation

Promoter

lacIq-Ptrc

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pNup133-Ypet

Plasmid

#98283

Purpose

mammalian expression of Nup133-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup133 (NUP133 Human)

Use

Tags

YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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PCTAIRE-1-HA

Plasmid

#1999

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

PCTAIRE-1 (CDK16 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
pMpGWB110

Plasmid

#68564

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pSNAPf-Nup43

Plasmid

#98276

Purpose

mammalian expression of SNAPf-Nup43

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup43 (NUP43 Human)

Use

Tags

SNAPf-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_1

Plasmid

#73525

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pCR1056

Plasmid

#71824

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hSAPS1-HA

Plasmid

#165466

Purpose

Human SAPS1 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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Puro-iDEST

Plasmid

#75336

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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