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Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-Gamillus
Plasmid#197920PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-Gamillus
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HsSmg6-mut-siRNAres_I
Plasmid#146587PurposeMammalian Expression of HsSmg6-mut-siRNAresDepositorInsertHsSmg6-mut-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1mut-siRNAres_E
Plasmid#146238PurposeInsect Expression of DmSMG6-PINNOB1mut-siRNAresDepositorInsertDmSMG6-PINNOB1mut-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1-siRNAres_E
Plasmid#146239PurposeInsect Expression of DmSMG6-PINNOB1-siRNAresDepositorInsertDmSMG6-PINNOB1-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSMG6-siRNAres-V5His6_E
Plasmid#146230PurposeInsect Expression of DmSMG6-siRNAresDepositorInsertDmSMG6-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pITC
Plasmid#192856PurposeCloning and expression of transcription factor gene under control of naringenin inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPfdeARAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
vgCam-pRSETb
Plasmid#193362PurposeA FRET-based calcium probe using SumireDepositorInsertvgCam
UseE.coli expressionTagsHis X6 tag and Strep tagExpressionMutationPromoterT7Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL-5.1
Plasmid#190589PurposeLentiviral vector-mediated delivery of artificial exons for protein tagging in mammalian cellsDepositorInsertsNeoR transgene
artificial exon encoding BSD and EGFP
UseLentiviralTagsExpressionMutationPromoterCellular and SV40Available sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpAGS-dGoLoco1-GFP
Plasmid#185577Purposefor overexpressionDepositorInsertAGS-dGoLoco1
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationdeletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTorD-H6
Plasmid#169039PurposeEscherichia coli TorD with C-terminal 6xHis in pET28a.DepositorInsertTorD-H6
UseTags6xHisExpressionBacterialMutationThe mutation N at position 46 was changed back to…PromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA3735
Plasmid#184848PurposegRNA cloning using blue-white screening for recombinantsDepositorInsertLacZalpha
UseCRISPRTagsExpressionMammalianMutationalpha-fragment of LacZPromoterU6Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
LDB34
Plasmid#185431PurposeYeast expression of Nup1 C-terminal truncation (nup1-21 allele)DepositorInsertNUP1
UseTagsExpressionYeastMutationTruncation at amino acid 1041 from the C-term of …PromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LDB33
Plasmid#185430PurposeYeast expression of Nup1 N-terminal truncation (nup1-15 allele)DepositorInsertNUP1
UseTagsExpressionYeastMutationTruncation of amino acids 3-191 from the N-term o…PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sp(AU)sgRNA-mKO2
Plasmid#184443PurposesgRNA with AU flip (sgRNA2.0), mKO2 as selection markerDepositorInsertsgRNA with AU flip, mKO2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Q0K750
Plasmid#163248PurposeInducible expression of UniProt identifier Q0K750DepositorInsertQ0K750
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_28_0570_WT
Plasmid#171642PurposeExpression of wildtype L. major glcyoprotein-63 from chromosome 28 in mammalian cellsDepositorInsertLmjF.28.0570
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_31_2000_WT
Plasmid#171643PurposeExpression of wildtype L. major glcyoprotein-63 from chromosome 31 in mammalian cellsDepositorInsertLmjF.31.2000
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PKM-Halo
Plasmid#175321PurposeProduction of HaloTag fusion proteinDepositorInsertPKM (PKM Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTN_Halo-STMN1
Plasmid#175299PurposeProduction of HaloTag fusion proteinDepositorInsertSTMN1 (STMN1 Human)
UseCell free expressionTags6xHis-HaloTagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only