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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Hsp68

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP3 C-V5-tag

Plasmid

#179962

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp3 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

Plasmid

#177320

Purpose

Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

Depositor

Xiaolin Nan, Steven Chu

Article

Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Insert

KRAS 4B (KRAS Human)

Use

Lentiviral

Tags

FKBP-derived dimerization domain (DD, or DmrB) an…

Expression

Mammalian

Mutation

G12D

Promoter

TetOn-CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

Plasmid

#172640

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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A*11:01:01:01

Plasmid

#203276

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS1089: mini-AAV.sgRNA.Nme2Cas9

Plasmid

#159536

Purpose

Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with single guide RNA cassette

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-EmGFP-LATS2/1 KD

Plasmid

#52085

Purpose

Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter

Depositor

Yutaka Hata

Article

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

LATS2 and LATS1 miRNAs (LATS1, LATS2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.KD.flag

Plasmid

#19759

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

D173A (kinase dead)

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only
pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

Plasmid

#172620

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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B*58:02:01

Plasmid

#203262

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*58:02:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*66:01:01

Plasmid

#203291

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*66:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

Plasmid

#172627

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1 +MIC

Plasmid

#158776

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-N-EP300

Plasmid

#166452

Purpose

Expresses fusion of mCherry and EP300-N-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, N-EP300(1-341) (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL A WT

Plasmid

#202413

Purpose

Expression of GFP-tagged PODXL (isoform A) WT

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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