Skip to main content
Addgene
Showing: 5821 - 5840 of 148587 results

  3. piRFP

    Plasmid
    #31857
    Purpose
    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging
    Depositor
    Vladislav Verkhusha
    Article
    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103
    Has Service
    Cloning Grade DNA
    Insert
    iRFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…
    Promoter
    CMV
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    31857-plasmid-map-sequence-id-396432

    Enlarge

  4. TA1-DuET

    Plasmid
    #213382
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TA1 (TAAR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213382-plasmid-map-sequence-id-430737

    Enlarge

  10. pIS1

    Plasmid
    #12179
    Depositor
    David Bartel
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12179-plasmid-map-sequence-id-234698

    Enlarge

  14. pICE-HA-MRE11-H129N

    Plasmid
    #82034
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Nuclease-dead mutant: H129N MRE11
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82034-plasmid-map-sequence-id-155387

    Enlarge

  16. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

    Enlarge

  17. LZRS-Mek2-K101A (KA7)

    Plasmid
    #21191
    Depositor
    Paul Khavari
    Insert
    Mek2 (MAP2K2 Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    K101A (catalytically inactive)
    Promoter
    Available since
    June 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  19. pFrt-invCAG-ires-Luc

    Plasmid
    #63576
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63576-plasmid-map-sequence-id-105724

    Enlarge

Showing: 5821 - 5840 of 148587 results