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Plasmid#176933PurposeExpresses PE2-NG variant in mammalian cellsDepositorInsertPE2-NG variant
UseLentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEFSAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA MOV10 K530A
Plasmid#51716Purposeplasmid expresses FLAG/HA-tagged MOV10 K530ADepositorInsertMOV10 K530A (MOV10 Human)
UseTagsFLAG/HAExpressionMammalianMutationK530APromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA MOV10 D645N
Plasmid#51717Purposeplasmid expresses FLAG/HA-tagged MOV10 D645NDepositorInsertMOV10 D645N (MOV10 Human)
UseTagsFLAG/HAExpressionMammalianMutationD645NPromoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
SMN2E7-Fluc-Nluc-reporter
Plasmid#221005PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.DepositorInsertFluc-SMN2E7-STOP-Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-WT
Plasmid#177614PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshiftingDepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-E12-vioABECD
Plasmid#66537PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)DepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterMutant T7 Promoter - G6 and Mutant T7 Promoter - …Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX_TRE3G_DD::mCherry
Plasmid#108679PurposeTunable system for controlled gene expression and protein stability in mammalian cellsDepositorInsertDestabilizing Domain (DD) mCherry fusion protein
UseLentiviralTagsDD::mCherry fusion proteinExpressionMammalianMutationPromoterDoxycycline inducibleAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorUseTagsExpressionMammalianMutationPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB_tetO7_sfGFP
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
UseTagsExpressionYeastMutationPromotertetO7 promoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF146 pSOD1WTAcGFP1
Plasmid#26407DepositorInsertSOD1 (SOD1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-G97 IRES EGFP
Plasmid#130711PurposePlasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-G97 IRES EGFP
UseTagsLyn11 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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SG3ΔENV CA E45A
Plasmid#145783PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residue glutamic acid 45 to alanin…PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV RT D185A
Plasmid#145782PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).DepositorInsertReverse Transcriptase
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Reverse Transcriptase Aspartic Acid 185 t…PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB-wt
Plasmid#58905Purposemammalian expression of wild type human RbDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA E28A,E29A
Plasmid#145785PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residues Glutamic Acid residues 28…PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-hGRK4
Plasmid#32690DepositorInsertGRK4 (GRK4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA Q63A,Q67A
Plasmid#145784PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residues Glutamines 63 and 67 to a…PromoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only