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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-DYRK1B_IDG-K

Plasmid

#158183

Purpose

Gateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-DYRK1B (DYRK1B Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn1-tdTomatof

Plasmid

#104060

Purpose

Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-eNpHR 3.0-EYFP

Plasmid

#26775

Purpose

3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Insert

eNpHR 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

hSyn

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRAS G12V-IRES-ASK1

Plasmid

#221074

Purpose

Expresses NRAS G12V and ASK1 in mammalian cells

Depositor

Hidenori Ichijo

Article

Takeru et al Communications Biology volume 7, Article

Inserts

NRAS (NRAS Human)
ASK1

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-WT

Plasmid

#25080

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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plk1 WT GFP-myc

Plasmid

#132963

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB103

Plasmid

#68557

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mNeonGreen-NLS-blast

Plasmid

#136258

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mNeonGreen-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB102

Plasmid

#68556

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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hIRE1a K599A

Plasmid

#20745

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

K599A, dominant negative kinase mutant

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
p667-UBC-BRSK2-V5-TurboID_IDG-K

Plasmid

#135253

Purpose

Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

BRSK2-V5-TurboID (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-TurboID-V5-BRSK2_IDG-K

Plasmid

#135272

Purpose

Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TurboID-V5-BRSK2 (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK12-V5_IDG-K

Plasmid

#135245

Purpose

Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK12-V5 (CDK12 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRN3P_EGFP_m6A_Tracer

Plasmid

#139403

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

m6A-Tracer

Use

In vitro transcription

Tags

EGFP

Expression

Mutation

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pExpreS2-2

Plasmid

#175362

Purpose

G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A

Plasmid

#8823

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A (GZMA Human)

Use

Tags

(His)6 and Enterokinase site

Expression

Bacterial

Mutation

it mature enzyme.

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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Cdk4

Plasmid

#1874

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-NCreERT2

Plasmid

#106370

Purpose

N-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

NCreERT2

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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