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CCR5-SZ190b-sfGFP

Plasmid

#162447

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-C chemokine receptor type 5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

truncation from aa88 to aa249

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PKM-IRES-hrGFP

Plasmid

#73405

Purpose

Mammalian expression of mouse PKM and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKM (Prkcz Mouse)

Use

Tags

3xFLAG and pIRES-hrGFP-2a

Expression

Mammalian

Mutation

truncated, constitutively active form

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pRetroX GFP Mps1 DK

Plasmid

#63703

Purpose

Inducible expression of GFP-Mps1 DK fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 47-156, kinetochore binding domain

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-SMAC-HA-eGFP

Plasmid

#67492

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Use

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP K45E

Plasmid

#21734

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Mutation of the ATP-binding site (K45E). This ver…

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pORANGE CACNG8-GFP KI #1

Plasmid

#131473

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV3

Plasmid

#78631

Purpose

GESTALT V3 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV4

Plasmid

#78630

Purpose

GESTALT V4 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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1xTRE DREaMR 3p3GFP

Plasmid

#182203

Purpose

1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)

Plasmid

#160634

Purpose

TU for wtGFP expression driven by 2x35s promoter.

Depositor

Diego Orzaez

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

2x35s

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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Cx43 delta dt GFP

Plasmid

#17671

Depositor

Cecilia Lo

Insert

Cx43 (Gja1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

a.a. 234-243 deleted

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pGFP-PTEN D92A

Plasmid

#50521

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

D92A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMT-GFP-p150Dynactin

Plasmid

#24283

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

p150 Dynactin (DCTN1-p150 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
EGFP-IC2-N237

Plasmid

#51410

Purpose

expression of GFP-tagged N-terminal AA 1-237 of dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AA 1-237

Promoter

CMV

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only
pSC-NF8-d1-eGFP

Plasmid

#122036

Purpose

AAV vector, NFkappaB-dependent eGFP expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al PLoS One. 2013;8(1):e53156. doi: 10.1371

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-eGFP

Plasmid

#126720

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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CAV1-mEGFP/FRT/TO

Plasmid

#27762

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only

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T7-His-GFP-Arp11

Plasmid

#51402

Purpose

expression of GFP-tagged mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
ATF4 13: 5’ATF4.uORF2AUA.GFP

Plasmid

#21860

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF2 was converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-iGFPx6

Plasmid

#105252

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Use

Tags

iGFPx6

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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