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Showing: 8701 - 8730 of 148587 results

  1. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

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  3. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  7. p5T7-LYCipi-ggpps

    Plasmid
    #122017
    Purpose
    Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Insert
    GGPP synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    codon optimized, truncated first 98 amino acids, …
    Promoter
    T7
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122017-plasmid-map-sequence-id-238964

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  10. BRD4

    Plasmid
    #38942
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38942-plasmid-map-sequence-id-348518

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  15. BM00654

    Plasmid
    #155339
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155339-plasmid-map-sequence-id-360426

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  23. p72B9-FBL-HC

    Plasmid
    #194637
    Purpose
    Expresses 72B9 hybridoma anti-FBL antibody, heavy chain
    Depositor
    Susan Baserga
    Insert
    72B9 antibody, heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    194637-plasmid-map-sequence-id-382496

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  24. p72B9-FBL-LC

    Plasmid
    #194638
    Purpose
    Expresses 72B9 hybridoma anti-FBL antibody, light chain
    Depositor
    Susan Baserga
    Insert
    72B9 antibody, light chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194638-plasmid-map-sequence-id-382497

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  26. pINDUCER20-FLAG-hPAX7 (#734)

    Plasmid
    #78339
    Purpose
    Doxycycline-inducible lentiviral overexpression of human PAX7 ORF with a FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    PAX7 (PAX7 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  30. pBlueScript-humanAPOBEC1

    Plasmid
    #134606
    Purpose
    Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    humanAPOBEC1 (APOBEC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Beta actin
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134606-plasmid-map-sequence-id-258758

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Showing: 8701 - 8730 of 148587 results