-
Plasmid#122596Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT13-splitD
Plasmid#122597Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG010
Plasmid#113984PurposeVector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertRK2 (IncP)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pOPS0314
Plasmid#115507PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celBDepositorInsertcelB pOGG050, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
UseTagsStrep II tag on Cas1ExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-AMOT p130 delta linker
Plasmid#32825DepositorInsertAMOT p130 (AMOT Human)
UseTagsHAExpressionMammalianMutationE5D and amino acids 752-1076 deletedPromoterCMVAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPA1L
Plasmid#19484DepositorInsertHSPA1L (HSPA1L Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-AMOT p130 deltaEYLI
Plasmid#32826DepositorInsertAMOT p130 (AMOT Human)
UseTags2xHAExpressionMammalianMutationE5D, deletion of last 4 amino acids (EYLI)PromoterCMVAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST QKI
Plasmid#19891DepositorInsertquaking homolog, KH domain RNA binding (QKI Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre
Plasmid#125574PurposeExpresses Cre under the CAG promoter in mammalian cells.DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP
Plasmid#199357PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-HRAS
Plasmid#80604PurposeMammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to itDepositorInsertHIV-1 p55 GAG
UseTagsExpressionMammalianMutationPromoterSRalphaAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2
Plasmid#79902PurposeGene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor LocusDepositorInsert3xFLAG_Twin_Strep_EZH2
UseCRISPR and TALENTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterPGK1Available sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-p300_Blast
Plasmid#192654Purpose3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance markerDepositorInsertVP64-dCas9-p300
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-iRFP670-GFP
Plasmid#111815PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
iRFP670
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT276
Plasmid#122607Purposeexpress CDA1-BE4max in mammalian cellsDepositorInsertCDA1-BE4max
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ZnF/HIT1
Plasmid#15332DepositorInsertzinc finger, HIT domain containing 1 (ZNHIT1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-syn pA
Plasmid#99689PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only