-
Plasmid#68826PurposeExpresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFPDepositorInserthuman RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12a-2AeGFP
Plasmid#123633PurposeMammalian expression, Genome editingDepositorInsertBsCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(flex2)
Plasmid#163431PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mlcR25-mEGFP
Plasmid#154295PurposeFluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)DepositorInsert25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
GET1_pcDNA6.2/EmGFP-Bsd
Plasmid#176985PurposeMammalian expression vector encoding GET1 and EmGFP-BsdDepositorInsertGET1 (GET1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
7xNLS-pMJ915v2-sfGFP
Plasmid#88922PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP-HF
Plasmid#157735Purposemammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP-HF
UseTagsHis8-FlagExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_AE
Plasmid#148577PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(R982A)
Plasmid#39487DepositorInsertARAP3(R982A) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationR982A = Rho GAP mutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mEGFP
Plasmid#168490PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.DepositorInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF1-GFP
Plasmid#67390PurposeMammalian expression of hArf1 with C-term GFP tagDepositorInsertARF1 (ARF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCUPI NPD-GFP
Plasmid#1191DepositorInsertN-terminus of Sup35 (SUP35 Budding Yeast)
UseCup1 promoterTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-EGFP-ccdB
Plasmid#14311DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSIF-H1-hpolb-copGFP
Plasmid#23256DepositorInsertDNA polymerase beta shRNA
UseLentiviral and RNAiTagsGFPExpressionMutationPromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
Nsp13 deltaN1-EGFP
Plasmid#165127Purposemammalian expression and localizationDepositorInsertNsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp13 deltaN2-EGFP
Plasmid#165128Purposemammalian expression and localizationDepositorInsertNsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-EGFP
Plasmid#14187DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Tubb3-4xGFP KI
Plasmid#183445PurposeGFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)DepositorInsertgRNA and 4xGFP donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt(R25C)
Plasmid#39532DepositorInsertAkt(R25C) (Akt1 Mouse)
UseTags6G linker, EGFP, and HAExpressionMammalianMutationR25CPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only