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Showing: 11561 - 11600 of 39906 results

  1. pCT03

    Plasmid
    #212150
    Purpose
    Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.
    Depositor
    Kang Zhou
    Insert
    alanine dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212150-plasmid-map-sequence-id-420600

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  2. pLV-SFFV-mCherry-WPRE-UbC-Emerald

    Plasmid
    #211797
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    mCherry
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211797-plasmid-map-sequence-id-418065

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  3. pPB-CAG-NPHP1

    Plasmid
    #209769
    Purpose
    Human NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    NPHP1 (NPHP1 Human, Synthetic)
    Use
    Piggybac transposon
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209769-plasmid-map-sequence-id-417213

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  4. pET24a MDH_CHLP8

    Plasmid
    #204242
    Purpose
    Bacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_CHLP8
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204242-plasmid-map-sequence-id-413340

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  8. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid
    #170788
    Purpose
    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Mortierella verticillate PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170788-plasmid-map-sequence-id-337769

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  9. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Use
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

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  12. pB2H12*

    Plasmid
    #163838
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12 (Y64A)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTPN12 Y64A
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163838-plasmid-map-sequence-id-337609

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  13. pB2H12

    Plasmid
    #163837
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTPN12 E57D
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163837-plasmid-map-sequence-id-337607

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  14. pB2H6*

    Plasmid
    #163836
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1 (R459M)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    SHP1 R459M
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163836-plasmid-map-sequence-id-337604

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  15. pB2Hopt*

    Plasmid
    #163831
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B(C215S)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTP1B C215S
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163831-plasmid-map-sequence-id-337592

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  20. pBW002

    Plasmid
    #131591
    Purpose
    Vector with aTc inducible M. laminosus Fd1 without BbsI restriction sites in the vector backbone
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    Mastigocladus laminosus ferredoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131591-plasmid-map-sequence-id-258983

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  21. pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo

    Plasmid
    #128454
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    128454-plasmid-map-sequence-id-256207

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  22. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

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  24. pACYCduet-mxLOX2-EH

    Plasmid
    #104979
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Inserts
    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    104979-plasmid-map-sequence-id-207891

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  25. pAM-35s-CIPK5-YFPn-1

    Plasmid
    #102392
    Purpose
    split YFP. Plant expression of CIPK5-YFPn-1
    Depositor
    Silke Robatzek
    Insert
    CIPK5 (CIPK5 Mustard Weed)
    Use
    Tags
    YFPn
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102392-plasmid-map-sequence-id-196617

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  26. pAM-35s-Snrk2.3-YFPn-1

    Plasmid
    #102391
    Purpose
    split YFP. Plant expression of Snrk2.3-YFPn-1
    Depositor
    Silke Robatzek
    Insert
    SNRK2.3 (SNRK2.3 Mustard Weed)
    Use
    Tags
    YFPn
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102391-plasmid-map-sequence-id-196616

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  27. pAM-35s-CIPK5-YFPc-1

    Plasmid
    #102387
    Purpose
    split YFP. Plant expression of CIPK5-YFPc-1
    Depositor
    Silke Robatzek
    Insert
    CIPK5 (CIPK5 Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102387-plasmid-map-sequence-id-195327

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  28. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72417-plasmid-map-sequence-id-135656

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  31. PP-LAMMER

    Plasmid
    #25292
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-LAMMER:D534-E875 (LAMMER Plasmodium falciparum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids D534-E875
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. PP-HSP90 (3IED)

    Plasmid
    #25293
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0417:S97-E347
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 97-347
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. PP-CDPK10

    Plasmid
    #25286
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd7_40:G126-S452 (cgd7_40 Cryptosporidium Parvum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids G126-S452
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. PP-PYRK1 (3GG8)

    Plasmid
    #25288
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    55.m00007:I39-E531
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids I39-E531
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. PP-CDPK6 (3IS5)

    Plasmid
    #25194
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    38.m00014:T112-A378
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids T112-A378
    Promoter
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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Showing: 11561 - 11600 of 39906 results