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Plasmid#208857PurposeSSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pHDR-PMG (pXK-1650)
Plasmid#208858PurposeHDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEQ1100
Plasmid#83937Purposeexpress eGFP with a C-terminal 6X his tag in eukaryotic cellsDepositorInsertEGFP
UseTags6xHIS, V5, and biotinylation signalExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ClopHensor-H148G-V224L
Plasmid#46366DepositorInsertClopHensor-H148G-V224L
UseTagsStrep-IIExpressionMammalianMutationE2GFP-H148G-V224L is EGFP-T203Y-H148G-V224LPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Po-Mt Short P2A
Plasmid#164116PurposeDetect the short Peroxisomes-mitochondria contactDepositorInsertSplit GFP Po-Mt Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Po-Mt Long P2A
Plasmid#164115PurposeDetect the long Peroxisomes-mitochondria contactDepositorInsertSplit GFP Po-Mt Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- Po Short P2A
Plasmid#164118PurposeDetect the short Peroxisomes-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP Mt-ER- Po Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- Po Long P2A
Plasmid#164117PurposeDetect the long Peroxisomes-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP Mt-ER- Po Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
FERM domain of Ezrin
Plasmid#62380PurposeExpresses the FERM domain of Ezrin (N terminal portion of the protein)DepositorInsertEzrin aa1-309 (EZR Human)
UseTagsGFPExpressionMammalianMutationcontains FERM domain of Ezrin (N terminal portion…PromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
hGFAP R239C
Plasmid#169476PurposeSingle p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAPDepositorInsertEGFP-hGFAP (GFAP Human)
UseTagsEGFPExpressionMammalianMutationChanged R 239 to CPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHLuc Control
Plasmid#141091PurposepH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEPDepositorInsertAntares-T2A-puromycin-IRESv24-eGFP-Nanoluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorInsertGFAP (Gfap Mouse)
UseTagsHaloTagExpressionMammalianMutationChanged R 236 to HPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- PM Long P2A
Plasmid#164109PurposeDetect the long Plasma membrane--mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP PM-Mt-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- PM Short P2A
Plasmid#164110PurposeDetect the short Plasma membrane-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP PM- Mt-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-2xMARS-PLD
Plasmid#205237PurposeExpresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMFDepositorInsertsUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
GA-NES
Plasmid#61018PurposeddGFP A-NESDepositorInsertddGFP A
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
TR therapy reporter
Plasmid#179083PurposeFluorescent reporter for alternative splicingDepositorInsertsDsRed1
EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mitoT
Plasmid#174519PurposeEngineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cellsDepositorInsertMitoT synthetic tether
UseTags6xHis and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB-NES
Plasmid#61017PurposeddGFP B-NESDepositorInsertddGFP B
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-optoB2-SS-YFP
Plasmid#75270PurposeArrestin independent version of optoB2 with serine mutations at S362A,S363GDepositorInsertArrestin independent version of optoB2
UseTagseGFPExpressionMammalianMutationserine mutations at S362A,S363GPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only