-
Plasmid#170280PurposeExpresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertUseTagsExpressionBacterialMutationPromoterTim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…Available sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pET9d-hnRNP-A1
Plasmid#23026DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1
Plasmid#83911Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB
Plasmid#79050PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-C
Plasmid#30224DepositorInsertrhVitronectin with C terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478PromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
pET28a-SpyTagMBP
Plasmid#35050DepositorInsertSpyTagMBP
UseTagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET11d-hUba2
Plasmid#53136PurposeE.coli expression of full length untagged human Uba2 (SAE2)DepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET50_WT MDA5
Plasmid#216802PurposeExpresses wild type (wt) melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_Nef_MCS2
Plasmid#66079PurposeSingle vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertNef
UseTags6xHis and HRV 3C SiteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21 E2F1
Plasmid#90313PurposeExpresses human E2F1 in bacteriaDepositorInsertE2F1 (E2F1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaA
Plasmid#50386PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.DepositorInsertPanK
UseTags6XHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b-KSRP
Plasmid#23019DepositorInsertKSRP (KHSRP Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PSAT1
Plasmid#83908Purposestable overexpressionDepositorInsertphosphoserine aminotransferase 1 (PSAT1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KIRIN1
Plasmid#107414PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1DepositorInsertKIRIN1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneUseTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-His
Plasmid#14030DepositorInsertCyPet
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN_FL
Plasmid#30223DepositorInsertrhVitronectin without deletions (VTN Human)
UseTagsExpressionBacterialMutationNo deletionsPromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-AsCas12f-WT
Plasmid#204640PurposeExpression of wild-type AsCas12f in E. coliDepositorInsertAsCas12f
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-T7-Ct-WspR
Plasmid#79166PurposeDual expression of Ct biosensor and WspR WT under individual T7 promotersDepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only