Skip to main content
Addgene
Showing: 561 - 580 of 13509 results

  6. eSrtA(4S-9) pET29b

    Plasmid
    #75146
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    N98D S102C A104V A118T F122A K134R F144L I182V E1…
    Promoter
    T7
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    75146-plasmid-map-sequence-id-142065

    Enlarge

  8. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

    Enlarge

  16. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Neil Olszewski
    Type
    Empty backbone
    Use
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84652-plasmid-map-sequence-id-162124

    Enlarge

  17. pET28a Gx-mNG-NL

    Plasmid
    #169106
    Purpose
    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - mNeonGreen - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169106-plasmid-map-sequence-id-335361

    Enlarge

  18. pCDB179-HotPETase

    Plasmid
    #218701
    Purpose
    Encodes codon optimized HotPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    HotPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218701-plasmid-map-sequence-id-434097

    Enlarge

  19. pCDB179-DepoPETase

    Plasmid
    #218702
    Purpose
    Encodes codon optimized DepoPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    DepoPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218702-plasmid-map-sequence-id-434087

    Enlarge

Showing: 561 - 580 of 13509 results