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Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationNote that insert length is based on sum of the 2 …PromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
PF4-bio-His
Plasmid#53411PurposeExpresses full-length Platelet factor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPF4 (PF4 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PEAR1-bio-His
Plasmid#51860PurposeExpresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPEAR1 (PEAR1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro2-UBXD1-mCherry
Plasmid#31835DepositorInsertUBXD1 (UBXN6 Human)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBCMV-MS2CP-eDHFR
Plasmid#167309PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertMS2CP-eDHFR
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAIP-hXRCC1pd-eGFP
Plasmid#206033PurposeMammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoterDepositorInserthXRCC1
UseTagseGFPExpressionMammalianMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterCAGAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mXRCC1pd-eGFP
Plasmid#206035PurposeMammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoterDepositorInsertmXRCC1pd
UseTagseGFPExpressionMammalianMutationS105A, S186A, K188A, S195A, S221A, S222A,S238A, S…PromoterEf1aAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyTagsExpressionBacterialMutationF11V, T13P, N39D, A179APromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry
Plasmid#182381PurposeDual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promotersDepositorInsertsUseTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterHSV TK promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
M51 Super 8x FOPFlash (TOPFlash mutant)
Plasmid#12457PurposeControl for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.DepositorInsertmutant TCF/LEF binding sites (Ctnnb1 )
UseLuciferaseTagsExpressionMammalianMutationmutant TCF/LEF binding sitesPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1 D256
Plasmid#12145DepositorInsertFoxo1 (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationContains a deletion truncating the protein after …PromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAmCyan1-C1-ADCY6
Plasmid#113905PurposeN-terminal Cyan Fluorescent tag in Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 (ADCY6 Human)
UseTagsCyanExpressionBacterial and MammalianMutationPromoterpCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK3-2A-GFP
Plasmid#118286PurposeExpresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)
UseAAVTags2A peptide and GFPExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S
Plasmid#62460Purposeexpresses C151S and C288S double mutant human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteines 151 and 288 of Keap1 to respect…PromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
FoxO1-ADA-GFP
Plasmid#35640DepositorInsertFoxO1-ADA-GFP (Foxo1 Mouse)
UseTagsFlag, GFP, and MycExpressionMammalianMutationT24A, S253D and S316A mutationsPromoterCMVAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1
Plasmid#159585PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-FKBP-LCa
Plasmid#59353PurposeExpresses human clathrin light chain A tagged with GFP & FKBP in mammalian cells.DepositorInsertClathrin Light Chain A (CLTA Human)
UseTagsEGFP and FKBPExpressionMammalianMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK2-2A-mCherry
Plasmid#118285PurposeExpresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
F5-bio-His
Plasmid#53426PurposeExpresses full-length Coagulation factor V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertF5 (F5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PVRL2-bio-His
Plasmid#51824PurposeExpresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPVRL2 (NECTIN2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only