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Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorHas ServiceCloning Grade DNAInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0
Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
Plasmid#61592PurposeA human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
VPS13C^mClover3
Plasmid#118760PurposeVPS13C internally tagged with mClover3 at residue 1914.DepositorInsertVPS13C (VPS13C Human)
UseTagsmClover3 inserted at position aa 1914ExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^mCherry
Plasmid#118758PurposeVPS13A internally tagged with mCherry at residue 1372.DepositorInsertVPS13A (VPS13A Human)
UseTagsmCherry inserted at position 1372ExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-Q61L
Plasmid#12986DepositorInsertCdc42 constitutively active (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-EGFP-Cdc42-wt
Plasmid#12975DepositorInsertCdc42 (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
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VPS13A^Halo
Plasmid#118759PurposeVPS13A internally tagged with Halo at residue 1372.DepositorInsertVPS13A (VPS13A Human)
UseTagsHalo tag inserted at position 1372ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDHblast-flx-sORF-Katushka
Plasmid#179457PurposeLentiviral vector encoding a CRE inducible Katushka geneDepositorInsertloxP-shuffledORF-loxP-Katushka
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1545_v1
Plasmid#126861Purpose4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1545_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS16 Pmyo-3 NPP-9 mChBioFLAG
Plasmid#45099DepositorUseTagsBLRP, FLAG, and mCherryExpressionWormMutationPromotermyo-3 and unc-119Available sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2
Plasmid#123618PurposeExpresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
L1E-1
Plasmid#21193DepositorUseRetroviralTagsExpressionMutationMek1 R4F fused to ER*, constitutive activePromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
CC2
Plasmid#21207DepositorUseSubclone vectorTagsExpressionMutationMek1 R4F fused to ER*, constitutive activePromoterAvailable sinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
DN9
Plasmid#21209DepositorUseSubclone vectorTagsExpressionMutationMek1 K97M fused to ER*, catalytically inactivePromoterAvailable sinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only