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Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2396-Tier1-PhCMV-4xCOX8_Ypet
Plasmid#169534PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).DepositorInsertPCMV-driven miochondria-localized yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-phaZ
Plasmid#169874PurposeSingle guide RNA plasmid for Cas9 targeting phaZ in P. putidaDepositorInsertsgRNA towards phaZ in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
NFkBr-IL1Ra
Plasmid#183065PurposeExpresses IL-1Ra downstream of a synthetic promoter activated by the NFkB signaling pathwayDepositorInsertinterleukin-1 receptor antagonist (Il1rn Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6L1TAG
Plasmid#158148PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertAga1p-FAPB2.3.6
UseTagsExpressionYeastMutationCloned in a TAG codon at the first position of th…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH72-Tier1-PhCMV-SEAP
Plasmid#169560PurposeTier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).DepositorInsertPCMV-driven human secreted placental alkaline phosphatase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2410-Tier1-PhCMV-Myr_mTagBFP2
Plasmid#169535PurposeTier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).DepositorInsertPCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHB-2
Plasmid#140955PurposeFor PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationR238S mutation in PhaCPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b_Qcat18
Plasmid#45146DepositorInsertE-coli-pathways-QconCAT18
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8CQ
Plasmid#52171PurposeEncodes module 8 with amino acids 12C-16Q for recognition of nt 1ADepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in module 8PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVH64-Tier1-PSV40-Citrine_p2a_SEAP
Plasmid#169558PurposeTier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).DepositorInsertPSV40-driven citrine-P2A-SEAP fusion construct
UseTagsExpressionMammalianMutationPromoterPSV40Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC017.109
Plasmid#171145PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS2374-Tier1-PhCMV-Gal4_VP16
Plasmid#169582PurposeTier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).DepositorInsertGAL4-transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p27(kip1)
Plasmid#50026PurposepENTR vector for human cyclin-dependent kinase inhibitor 1BDepositorInsertp27(kip1) (CDKN1B Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS2163
Plasmid#169632PurposeTier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)DepositorInsertOTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
UseTagsExpressionMammalianMutationPromotertetO7-PhCMVmin / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only