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pB80-FKBP-mCherry-RAB11

Plasmid

#174622

Purpose

Rapalog-inducible coupling to recycling endosomes (RAB11) via FKBP

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

FKBP-mCherry-RAB11 (RAB11A Human, Synthetic)

Use

Tags

FKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB11: Met1Del

Promoter

Chicken beta-actin

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-p63RhoGEF

Plasmid

#67896

Purpose

Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-p63RhoGEF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-GFP-HDEL

Plasmid

#21771

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 2001 Apr 2. 153(1):47-62.

Insert

Green Fluorescent Protein (GFP)

Use

Tags

HDEL and preKar2

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mCit-IRES-Neo

Plasmid

#155230

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mCitrine sensor

Use

Lentiviral

Tags

mCitrine

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Expression

Mutation

Promoter

Beta Actin

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Cherry-Plekhh1

Plasmid

#187372

Purpose

Expresses human Plekhh1 labelled with Cherry

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.NgCAM-Halo

Plasmid

#162719

Purpose

Mammalian expression of chicken NgCam

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

NgCam (L1CAM Chicken)

Use

Tags

Halo

Expression

Mammalian

Mutation

E137K, K138E, and K642E

Promoter

Chicken Beta actin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pmCIT-CFPGgVcl 1-419 control 2 FRET

Plasmid

#46283

Depositor

Susan Craig

Article

Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-9

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato mouse Rab35 WT

Plasmid

#53514

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pmCherry-C1-DH

Plasmid

#67898

Purpose

Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-DH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST HRP Rab35 WT

Plasmid

#53498

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

HRP

Expression

Insect

Mutation

Promoter

GAL4

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato mouse Rab35 DN

Plasmid

#53513

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Tags

tdtomato

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP mouse Rab35 WT

Plasmid

#46014

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pUAST YFP mouse Rab35 DN

Plasmid

#46016

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S22N (dominant negative)

Promoter

GAL4

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_UG7

Plasmid

#135990

Purpose

Expresses UG7 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH

Use

Tags

helical linker-mEGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST myc mouse Rab35 CA

Plasmid

#53516

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Tags

myc

Expression

Insect

Mutation

Q67L

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
hs-Cap2

Plasmid

#41972

Depositor

Norbert Perrimon

Article

Baum et al Nat Cell Biol. 2001 Oct;3(10):883-90.

Insert

Cap2 (capt Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato Rab35 DN

Plasmid

#53499

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

tdtomato

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato Rab35 WT

Plasmid

#53502

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab35 DN

Plasmid

#53501

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only