Showing: 561 - 580 of 933 results
-
Plasmid#123931PurposeEncodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPhic31attB tagBFP destination vector
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MTK0_017
Plasmid#123932PurposeEncodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertBxBI attB tagBFP destination vector
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSARGENT1
Plasmid#216315PurposeReporter cassette for SARGENT with piggyBac ITRs and 10x Capture SequenceDepositorInserttagBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab4a-c-Myc
Plasmid#79799PurposeExpress TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).DepositorInsertRab4a (RAB4A Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pTag-BFP-C-h-Rab5a-c-Myc
Plasmid#79801PurposeExpress TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).DepositorInsertRab5a (RAB5A Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-dCas9-BFP-KRAB
Plasmid#221502PurposeCRISPRi machinery fish codon optimizedDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionTagsExpressionMutationD10A and H839A point mutationsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRDA174_EF1a_EnAsCas12a_BFP
Plasmid#196724PurposeSortable and selectable EnAsCas12a.DepositorInsertenASCas12a-HA-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS - HA and SV40 NLSExpressionMutationE174R, S542R, K548RPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pHR Ef1a-dCas9-BFP-KRAB
Plasmid#217304Purposelentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoterDepositorInsertdCas9-tagBFP-KRAB
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMutationPromoterEf1alphaAvailabilityAcademic Institutions and Nonprofits only -
lenti-RfxCas13d-Blast-BFP
Plasmid#196726PurposeSortable and selectable RfxCas13d.DepositorInsertRfxCas13d-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsSV40 NLSExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pUbi_dCas9-BFP-KRAB-gRNA
Plasmid#221503PurposeCRISPRi machinery fish codon optimized expressing plasmidDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionTagsExpressionMutationD10A and H839A point mutationsPromoterZebrafish Ubiquitin promoterAvailabilityAcademic Institutions and Nonprofits only -
pQCXIH FRB-BFP
Plasmid#202425PurposeTagBFP FKBP-rapamycin binding (FRB) dimerization domainDepositorInsertFKBP-rapamycin binding (FRB) dimerization domain
UseLentiviralTagsTagBFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pQCXIH FRB Ubiqutin-BFP
Plasmid#202426PurposeTagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitinDepositorInsertFKBP-rapamycin binding (FRB) dimerization domain
UseLentiviralTagsUbiquitin, TagBFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-BTRC-MGMT_2
Plasmid#136416PurposeLentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(BTRC)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-CTBP2-MGMT_1
Plasmid#136419PurposeLentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP8.1
Plasmid#189846PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.DepositorTypeEmpty backboneUseCre/LoxTagsmRFP and tagBFPExpressionMutationPromoterbeta ActinAvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP8.3
Plasmid#189848PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.DepositorTypeEmpty backboneUseCre/LoxTagsmRFP and tagBFPExpressionMutationPromoterbeta ActinAvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-Exo-Blast-BFP
Plasmid#196721PurposeSortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.DepositorInsertCas9-Exo1-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
tRECs/3xUAS-FP attB
Plasmid#220330Purposeencodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)DepositorInsertsB3RT-STOP-B3RT-TagBFP-3xHA
KDRT-STOP-KDRT-GFP-HSV
FRTm71-STOP-FRTm71-mCherry
UseTagsExpressionInsectMutationPromoterUASAvailabilityAcademic Institutions and Nonprofits only -
pHR hEF1A-BFP-P2A-rtTA-WPRE
Plasmid#223288PurposeA lentiviral vector expressing BFP and the TetON transcription factor rtTA.DepositorInsertBFP-P2A-rtTA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 561 - 580 of 933 results