Skip to main content
Addgene
Showing: 561 - 580 of 826 results

  1. pE2F5.1.0-gDNA

    Plasmid
    #112447
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5
    Depositor
    Kevin White
    Insert
    E2F5 (E2F5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112447-plasmid-map-sequence-id-230459

    Enlarge

  2. pDLX1.1.0-gDNA

    Plasmid
    #112439
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DLX1
    Depositor
    Kevin White
    Insert
    DLX1 (DLX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112439-plasmid-map-sequence-id-230396

    Enlarge

  3. pHBP1.1.0-gDNA

    Plasmid
    #112432
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HBP1
    Depositor
    Kevin White
    Insert
    HBP1 (HBP1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112432-plasmid-map-sequence-id-230372

    Enlarge

  4. pATF1.1.0-gDNA

    Plasmid
    #112424
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF1
    Depositor
    Kevin White
    Insert
    ATF1 (ATF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112424-plasmid-map-sequence-id-230304

    Enlarge

  7. PX852

    Plasmid
    #62884
    Purpose
    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    62884-plasmid-map-sequence-id-105906

    Enlarge

  9. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

    Enlarge

  11. PX854

    Plasmid
    #62886
    Purpose
    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa573-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62886-plasmid-map-sequence-id-105909

    Enlarge

  12. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

    Enlarge

  13. pARNTL.1.0-gDNA

    Plasmid
    #112480
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL
    Depositor
    Kevin White
    Insert
    ARNTL (BMAL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112480-plasmid-map-sequence-id-230516

    Enlarge

  14. pHEY1.1.0-gDNA

    Plasmid
    #112393
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1
    Depositor
    Kevin White
    Insert
    HEY1 (HEY1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112393-plasmid-map-sequence-id-230239

    Enlarge

  15. pDDIT3.1.0-gDNA

    Plasmid
    #112396
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DDIT3
    Depositor
    Kevin White
    Insert
    DDIT3 (DDIT3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112396-plasmid-map-sequence-id-230243

    Enlarge

  16. pTFAP2C.1.0-gDNA

    Plasmid
    #113792
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2C
    Depositor
    Kevin White
    Insert
    TFAP2C (TFAP2C Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113792-plasmid-map-sequence-id-241260

    Enlarge

  17. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62888-plasmid-map-sequence-id-105911

    Enlarge

  18. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    62887-plasmid-map-sequence-id-105910

    Enlarge

  20. bbCas9pluspAAA

    Plasmid
    #82581
    Purpose
    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
    Depositor
    Ivo Huijbers
    Article
    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:
    Insert
    Cas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    82581-plasmid-map-sequence-id-228465

    Enlarge

Showing: 561 - 580 of 826 results