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Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFLtail
Plasmid#232055PurposeExpresses construct for surface tethering: R. norvegicus NFL tail domainDepositorInsertNeurofilament-Light tail domain
UseTags6xHisExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf1
Plasmid#232058PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1DepositorInsertNeurofilament-Medium tail domain, charge shuffle 1
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf2
Plasmid#232059PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2DepositorInsertNeurofilament-Medium tail domain, charge shuffle 2
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-CC-S2
Plasmid#233243PurposeBacterial expression of Avi-CC-S2DepositorInsertAviTag-CC-S2
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28A-Rab8A
Plasmid#228880PurposeExpression of His-tagged Rab8A in E. coliDepositorInsertRAB8A (RAB8A Human)
UseTagsHis tagExpressionBacterialMutationresidues 1-6 and 177-207 missingPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28_pScaC(50-159)
Plasmid#228836PurposeE. coli expression of a truncated ScaC passenger domain (aa 50-159)DepositorInsertScaC
UseTagsStrepExpressionBacterialMutationaa 50-159PromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaQ
Plasmid#224429PurposeExpress AzaQ in E. coliDepositorInsertazaQ
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelG
Plasmid#225729PurposeTo express MelG (Rv1937) in E. coliDepositorInsertmelG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-VapBC35
Plasmid#225733PurposeTo express VapBC35(Rv1962) Toxin antitoxin in E. coliDepositorInsertvapBC35
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-T-Lg
Plasmid#216977PurposeExpression of GvpT-Linker-Large BiTDepositorInsertGvpT-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b_pScaC-SNAP_6xHis
Plasmid#228835PurposeE. coli expression of the SNAP-tagged ScaC passenger domainDepositorInsertScaC
UseTagsSNAPtag-TEV-6xHisExpressionBacterialMutationaa 33-223PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII
Plasmid#225075PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b-T4gp45
Plasmid#228204PurposeExpression in BL21(DE3) of WT T4 clampDepositorInsert45 (45 Escherichia phage T4)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-H-V5-His
Plasmid#222850PurposeE. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CsHPAD-AE
Plasmid#226982PurposeExpressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coliDepositorInsertCsHPAD-AE
UseTagsHis6 (on backbone)ExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD-AE
Plasmid#226968PurposeExpressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coliDepositorInsertOuIAD-AE
UseTagsHis6 (on backbone)ExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only