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LIC4B-His-TEV-TAK1-TAB1

Plasmid

#154874

Purpose

Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

Depositor

Kenneth Westover

Article

Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

Insert

TAK1-TAB1 (TAB1 Human)

Use

Tags

LIC TEV His

Expression

Insect

Mutation

Codon optimized

Promoter

polyhedrin

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MKK1K97A

Plasmid

#107974

Purpose

expression of a dominant negative MAP Kinase Kinase

Depositor

Edwin Krebs

Article

Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

Insert

MKK1 K97A (MAP2K1 Human)

Use

Tags

Expression

Mammalian

Mutation

dominant negative; see Depositor Comments below

Promoter

CMV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-CAMK2A

Plasmid

#23408

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK2A (CAMK2A Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP3K7

Plasmid

#23693

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K7 (MAP3K7 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP3K11

Plasmid

#23473

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K11 (MAP3K11 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP3K5

Plasmid

#23517

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K5 (MAP3K5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K12

Plasmid

#23626

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K12 (MAP3K12 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K2

Plasmid

#23454

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K2 (MAP3K2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K14

Plasmid

#23732

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K14 (MAP3K14 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K6

Plasmid

#23853

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K6 (MAP3K6 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMT2-MEKK3-K392A

Plasmid

#21585

Depositor

John Kyriakis

Article

Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

Insert

mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)

Use

Tags

Expression

Mammalian

Mutation

Lysine 392 to Arginine

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K8

Plasmid

#23538

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K8 (MAP3K8 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

1MM

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-PSK2 (K57A)

Plasmid

#197118

Purpose

Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (K57A) (TAOK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-tagged PSK (K57A)

Plasmid

#197150

Purpose

Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7 K64M

Plasmid

#75143

Purpose

Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lys 64 mutated to Met; kinase inactive and F236L …

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PBK

Plasmid

#23782

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PBK (PBK Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MKNK1

Plasmid

#23531

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MKNK1 (MKNK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MKNK2

Plasmid

#23735

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MKNK2 (MKNK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC649288

Plasmid

#23391

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC649288 (AK4P6 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pJExpress414_GmPOPB

Plasmid

#92234

Purpose

Express wild type prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

none

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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