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Plasmid#38041DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterCAAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only
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LvPolyUb
Plasmid#218982PurposeL.variegatus polyubiquitin-C promoterDepositorInsertL.variegatus polyubiquitinC promoter
UseSea urchinTagsExpressionMutationPromoterL.variegatus polyubiquitinC promoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-12Pk-hphMX6
Plasmid#49177Purposegenomic 12Pk epitope tagging in S. pombeDepositorTypeEmpty backboneUseTags12PkExpressionYeastMutationPromoterAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G9-5FLAG-hphMX6
Plasmid#49185Purposegenomic G9-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneUseTagsG9-5FLAGExpressionYeastMutationPromoterAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-LEU2MX6
Plasmid#33138Purposegene deletion in S. cerevisiae and S. pombeDepositorInsertLEU2MX6
UseTagsExpressionMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-12Pk-kanMX6
Plasmid#49176Purposegenomic 12Pk epitope tagging in S. pombeDepositorTypeEmpty backboneUseTags12PkExpressionYeastMutationPromoterAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-12Pk-LEU2MX6
Plasmid#49179Purposegenomic 12Pk epitope tagging in S. pombeDepositorTypeEmpty backboneUseTags12PkExpressionYeastMutationPromoterAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-12Pk-ura4MX6
Plasmid#49180Purposegenomic 12Pk epitope tagging in S. pombeDepositorTypeEmpty backboneUseTags12PkExpressionYeastMutationPromoterAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G9-5FLAG-kanMX6
Plasmid#49181Purposegenomic G9-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneUseTagsG9-5FLAGExpressionYeastMutationPromoterAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G11-5FLAG-kanMX6
Plasmid#49182Purposegenomic G11-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneUseTagsG11-5FLAGExpressionYeastMutationPromoterAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX-RG
Plasmid#38043DepositorInsertRabies virus glycoprotein
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCSCX
Plasmid#63918PurposeExpression of the sucrose utilization phenotypeDepositorInsertcscAKB
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCVD437
Plasmid#63758Purpose7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)DepositorInserteae
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pBpF
Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32G, E107P, D158T, I159SPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetO(3G)-WPRE
Plasmid#186206PurposetTA dependent gene expression vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterTetO(3G)Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
UseTagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-OH
Plasmid#138956PurposeUsed to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv with C terminal epitope tag (kkv Fly)
UseTagsollas and his-6 added to C terminus of protein co…ExpressionInsectMutationPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-kkv-3::NG
Plasmid#138960PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.DepositorArticleInsert3' end of kkv and neon green (kkv Fly)
UseAn editing vector for drosophilaTagsNeon Green fused to C terminus of KkvExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-154
Plasmid#166230Purposelenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherryDepositorInsertsU6-gRNA EGFP targetting
nCas9-PmCDA1-UGI-2A-mCherry
UseCRISPR and LentiviralTagsExpressionMutationPromoterCMV promoter and Human U6Available sinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetO(3G)-GCaMP6f-WPRE
Plasmid#186205PurposeExpress GCaMP6f under TetO(3G)DepositorInsertGCaMP6f
UseAAVTags6xHis (N terminal on insert), T7 epitope, and Xpr…ExpressionMutationPromoterTetO(3G)Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ spike_del19
Plasmid#155297PurposeExpression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particlesDepositorInsertSARS-Cov2 spike_deleted (S SARS-Cov2)
UseGeneration of sars-cov-2-pseudotyped lentiviral p…TagsExpressionMutationDeletion in C-term 19 aa of spikePromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMVA2RBS035
Plasmid#121051PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
UseTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMVA2
Plasmid#119951PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
UseTagsExpressionBacterialMutationA110GPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-c-Myc-Ubc-rtTA
Plasmid#198190PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthC-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-BMI1-Ubc-rtTA
Plasmid#198191PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthBMI1 (BMI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-BclXL-Ubc-rtTA
Plasmid#198189PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthBCLXL (BCL2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NI_G1333-DddA11-N
Plasmid#191609PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NN_G1333-DddA11-N
Plasmid#191610PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only