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Plasmid#188991PurposeA plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK1221
Plasmid#84220PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherryDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA
Plasmid#37087PurposeExpresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Medium-FT
Plasmid#31917DepositorInsertMedium-FT
UseTagsHisExpressionBacterialMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAGImC_Empty
Plasmid#92015PurposeEmpty backbone, includes IRES-mCherryDepositorTypeEmpty backboneUseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSin wPGK-Cre
Plasmid#101242Purposelow-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expressionDepositorInsertCre recombinase
UseLentiviralTagsExpressionMutationsee attached filePromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX
Plasmid#188992PurposeA plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:RFP
Plasmid#108455PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertTol2-Amp-y-cryst-mCherry
UseGatewayTagsExpressionMutationPromoterAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.4t
Plasmid#210256PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCherry
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA
Plasmid#184633PurposeExpresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertAldh1a1 (Aldh1a1 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA
Plasmid#37756PurposeExpresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX
Plasmid#188993PurposeA plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationD201G, A258T, K327R, G328S, G381V, G429D, T450APromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-PMG (pXK-1649)
Plasmid#208857PurposeSSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA
Plasmid#37086PurposeExpresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-YFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
S12.1_Empty_Bridge
Plasmid#127670PurposeAmpicillin resistant multiplexing backbone without CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP-LUC
Plasmid#60976PurposeRapid selection of recombinant marker-free bioluminescent rodent malaria parasitesDepositorInsertsGFP-Luciferase fusion
mCherry
hDHFR-yFCU
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-BclXL-Blasticidin
Plasmid#120718PurposeExpresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertBl2-like protein 1, isoform Bcl-X(L)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutation33 c-terminal amino acidsPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-2xStop
Plasmid#164412PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only