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pcDNA3.1 Hmga2 tr

Plasmid

#14793

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 truncated (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

truncataed HMGA2. As in human tumors, Hmga2-tr la…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pPPAT-2XStrep

Plasmid

#125620

Purpose

expresses human PPAT protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PPAT (PPAT Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgs2_Balbc

Plasmid

#170301

Purpose

A knock-out vector for the mouse Ptgs2 in Balb/c

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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alpha1Ddelta-G12-FRB

Plasmid

#87454

Purpose

rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

a1D short variants (Cacna1d Rat)

Use

Tags

FRB

Expression

Mammalian

Mutation

delete KYPAKNTTIALQMLERML

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4-hB90

Plasmid

#18968

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican C-terminus (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Human brevican signal peptide aa 1-23 followed by…

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pPFAS-2XStrep

Plasmid

#125682

Purpose

expresses human PFAS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PFAS (PFAS Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP1-His

Plasmid

#67869

Purpose

bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP1 (PRORP1 Mustard Weed)

Use

Tags

His

Expression

Bacterial

Mutation

Residues 36-572 (see comments)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pBCMV-MS2CP-eDHFR

Plasmid

#167309

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

MS2CP-eDHFR

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2CP-VPg(FCV)

Plasmid

#167314

Purpose

Template pDNA to prepare CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE H-Ras 61L, 186S

Plasmid

#14719

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

H-ras (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

61L, 186S

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pCGN H-Ras 61L, 189L

Plasmid

#14721

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

H-ras (HRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

61L, 189L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pBABE H-Ras 12V, 189L

Plasmid

#14718

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

H-ras (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V, 189L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-LKB1(S431A)

Plasmid

#84641

Purpose

mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(S431A) (STK11 Human, Synthetic)

Use

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

PKA phosphorylation site Serine 428 mutated to Al…

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-PmNG

Plasmid

#232201

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP Keima-PEX26

Plasmid

#224557

Purpose

expresses Keima fused PEX26 peroxisomal localization sequence in mammalian cells

Depositor

Gregory Wyant, William Kaelin

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

Keima-PEX26 (PEX26 Human)

Use

Retroviral

Tags

Keima

Expression

Mammalian

Mutation

PEX26 peroxisomal targeting sequence

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP APEX-PEX26

Plasmid

#224562

Purpose

expresses APEX fused to PEX26 peroxisomal localization sequence in mammalian cells

Depositor

Gregory Wyant, William Kaelin

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

PEX26 (PEX26 Human)

Use

Retroviral

Tags

APEX

Expression

Mammalian

Mutation

PEX26 peroxisomal targeting sequence

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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SQ171fg/pCSacB

Plasmid

#69344

Purpose

SQ171 fast growing strain supported by pCSacB plasmid

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

pCSacB

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Q79-GFP

Plasmid

#21159

Depositor

Junying Yuan

Article

Sanchez et al Nature. 2003 Jan 23. 421(6921):373-9.

Insert

HA-Q79-GFP

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pSBbi-moxGFP-P2A-T2A-Puro

Plasmid

#176893

Purpose

Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

Depositor

Jorge Luis Arias-Arias

Article

Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

Insert

moxGFP-P2A-T2A

Use

Transposon

Tags

moxGFP

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH-TAZ S89A

Plasmid

#179910

Purpose

Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Insert

WW Domain Containing Transcription Regulator 1 (WWTR1 Human)

Use

Tags

3X Flag-tag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Promoter

EF1a

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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