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Plasmid#16226DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutation2SA: S97A, S101APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mEGFP
Plasmid#105794PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
TfRΔEctoR1-eGFP A206K
Plasmid#113554PurposeTransmembrane model receptor to probe receptor internalization via clathrin mediated pathwayDepositorInsertTfRΔEctoR1-eGFP A206K
UseTagsExpressionMammalianMutationeGFP A206KPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300E/S302D
Plasmid#62531PurposeSerine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acidDepositorInserthuman Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to glutamic acid and serine 3…PromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFYF1328 EGFP Site#3
Plasmid#47513Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 3
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-EGFP
Plasmid#14190DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP-ccdB
Plasmid#14315DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJH26_pBad_AraC_Para-sfGFP.1TAG
Plasmid#163721PurposeArabinose-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertaraC gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH27_pBad_AraC_Para-sfGFP.2TAG
Plasmid#163722PurposeArabinose-inducible expression of green fluorescent protein encoding two amber codonsDepositorInsertaraC gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH25_pBad_AraC_Para-sfGFP.0TAG
Plasmid#163720PurposeArabinose-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertaraC gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 delta2-29
Plasmid#124923PurposeFor expression of a.a.2-29 deletion mutant of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
UseTagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-Cep123-C2 domain
Plasmid#73329PurposeEncodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFPDepositorInsertCep123 (CEP89 Human)
UseTagsEGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS16-iGFPx6
Plasmid#105275PurposePlasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.DepositorInsertVps16 portion (VPS16 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(flex0)
Plasmid#163429PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 5 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcherEQ-sfGFP
Plasmid#107421PurposeFor production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
UseTagsHis tag, StrepII, and superfolder GFPExpressionBacterialMutationmutation of glutamate 77 to glutaminePromoterTetAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-EGFP-ccdB
Plasmid#14309DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GSG1l-GFP KI
Plasmid#131492PurposeEndogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)DepositorInsertgRNA and GFP donor (Gsg1l Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS316-GFP-COF1
Plasmid#31843DepositorInsertCofilin (COF1 Budding Yeast)
UseTagsGFP (S65T)ExpressionYeastMutationPromoterT7Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
Trip-GFP-hOCLN-ΔN
Plasmid#32792DepositorInsertOCLN (OCLN Human)
UseLentiviralTagsGFPExpressionMammalianMutationDeletion of aa 1-63PromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-J23101-GFPuv
Plasmid#106390PurposepBBR6k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only