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Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas7c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CAG.Pro
Plasmid#79490PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoterDepositorInsertCAG Promoter
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCAG PromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
JH03
Plasmid#73512PurposemKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2DepositorInsertpmex-5::mKate2::PH::tbb-2
UseTagsmKate2ExpressionMutationPromotermex-5Available sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPS0750
Plasmid#115511PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.DepositorInsertgusA pOGG083, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRY2PHR-PSD95FingR-GFP
Plasmid#89443PurposeLocalizes CRY2 to excitatory synapsesDepositorInsertCRY2-PSD95FingR-GFP
UseTagsExpressionMammalianMutationContains CRY2 a.a. 1-498PromoterCAGAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ULK1 (836-1050)-MBP
Plasmid#203562PurposeMammalian expression of ULK1 (836-1050) with MBP tagDepositorInsertULK1 (ULK1 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-AsCas12f-v4.1
Plasmid#204636PurposeExpression of AsCas12f v4.1 in mammalian cellsDepositorInsertAsCas12f-v4.1
UseTagsNLSExpressionMammalianMutationD169K, N199K, N328G, D364RPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT29-splitC
Plasmid#122598Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3
Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT371
Plasmid#125612PurposeExpresses CDA1-BE4max-NG in mammalian cellsDepositorInsertCDA1-BE4max-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneUseTagsGal4 Activation domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETCON-TNF
Plasmid#67748PurposeTumor Necrosis Factor Alpha Yeast Display PlasmidDepositorInsertTNF-Alpha
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-mCherry
Plasmid#79509PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDSDepositorInsertmCherry RFP
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FSW-HRP-V5-NLGN2
Plasmid#82545PurposeOverexpression vector for V5-NLGN2 in neuronsDepositorInsertNLGN2 (Nlgn2 Rat)
UseLentiviralTagsHRP and V5ExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAV1-P132L-mEGFP
Plasmid#27708DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationP132LPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pT7-eGFP
Plasmid#32552DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterpT7Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TCF7
Plasmid#79498PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDSDepositorInsertTCF7 (TCF7 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsUPF2-siRNAres_I
Plasmid#146567PurposeMammalian Expression of HsUPF2-siRNAresDepositorInsertHsUPF2-siRNAres (UPF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pYPQ141-ZmUbi-RZ-Cas12j2
Plasmid#173926PurposeGateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only