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Plasmid#119164PurposeFusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.DepositorInsertHyPer-DAAO-NES
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKC-4.04
Plasmid#112085PurposeControl for NMD sensor: pCMV-3XFLAG-Fluc-betaglobinDepositorInsert3XFLAG firefly luciferase-beta-globin (HBB Human, Photinus pyralis, Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKC-4.06
Plasmid#112084PurposeNMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)DepositorInsert3XFLAG firefly luciferase-beta-globin 39PTC (HBB Human, Photinus pyralis, Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNLF1W
Plasmid#136404PurposeExpression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.DepositorTypeEmpty backboneUseLuciferaseTagsNanoLuc luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD3
Plasmid#20185DepositorInsertNotch 3 Intracellular Domain (Notch3 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 3 Intracellular Domain (Met…PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZW6 (CK2alpha)
Plasmid#27086DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SypHer2-DAAO-NES
Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATGL*-V5-APEX2
Plasmid#170574PurposeA C-terminal APEX2 fusion to the human ATGL S47A proteinDepositorInsertATGL* (PNPLA2 Human)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationchanged serine 47 to alaninePromoterCMVAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 RPEL1-EGFP-3XNLS
Plasmid#58469PurposeExpresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoterDepositorInsertsUseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
UseTagsDHFR and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD4
Plasmid#20186DepositorInsertNotch 4 Intracellular Domain (Notch4 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 4 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CSNK1A1 WT-Puro
Plasmid#123319PurposeLentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJPT018-LAT_WT-HaloTag-3x FLAG
Plasmid#202502PurposeExpress WT-LATDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-EGFP
Plasmid#65258Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_RV6_empty
Plasmid#203811PurposePlasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorUseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
msfGFP-Cx43
Plasmid#69024PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cx26-msfGFP
Plasmid#69016PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx26 (Gjb2 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only