Addgene

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pCDNA3 T7 BimL (S44A)

Plasmid

#24245

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed Serine 44 to Alanine

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163029

Purpose

FLiCRE TF for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-hLOV1-TEVcs-tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SFFV-NFE2L1-Puro

Plasmid

#181917

Purpose

Lentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L1 (NFE2L1 Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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plenti6-SLC7A11/xCT-V5

Plasmid

#170427

Purpose

Eepresses SLC7A11 (also named xCT) in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcVpx.myc

Plasmid

#213388

Purpose

Generate lentiviral vector which increases gene delivery into primary human myeloid cells

Depositor

Nathaniel Landau

Article

Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

Insert

SIVmac vpx (vpx Simian Immunodeficiency Virus)

Use

Lentiviral

Tags

myc-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-V5-TurboID(N)-GPI

Plasmid

#163696

Purpose

Expresses n-terminal half of TurboID on surface of neurons

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

Plasmid

#163697

Purpose

Expresses c-terminal half of TurboID on surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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BSD-CryC

Plasmid

#209672

Purpose

Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

Depositor

Sanjeevi Sivasankar

Article

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

CRY2 (CRY2 Mustard Weed)

Use

Lentiviral

Tags

Split-TurboID-C and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G BCL2L11 Neo

Plasmid

#140747

Purpose

Inducibly expresses BIM in mammalian cells

Depositor

Scott Dixon

Article

Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

Insert

BCL2L11 (BCL2L11 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV TRE3G

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB

Plasmid

#79050

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Article

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Expression

Mutation

Promoter

MSCV

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

Plasmid

#129632

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXM1

Plasmid

#153136

Purpose

Expression of Flag tagged human FOXM1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXM1 (FOXM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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AF1521-c003

Plasmid

#173111

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Use

Tags

Nanoluc

Expression

Bacterial

Mutation

KH-M1-L192

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163031

Purpose

FLiCRE TF with fast kinetics for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-f-hLOV1-TEVcs-tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK

Plasmid

#133080

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full length

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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