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Plasmid#193079Purposeconstitutive expression of mCherry fused to human Gata2 with mutation L359V in mammalian cellsDepositorInsertmCh-GATA2-L359V
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R361L
Plasmid#193080Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R361L in mammalian cellsDepositorInsertmCh-GATA2-R361L
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R362Q
Plasmid#193081Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R362Q in mammalian cellsDepositorInsertmCh-GATA2-R362Q
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-C373R
Plasmid#193082Purposeconstitutive expression of mCherry fused to human Gata2 with mutation C373R in mammalian cellsDepositorInsertmCh-GATA2-C373R
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R396Q
Plasmid#193083Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R396Q in mammalian cellsDepositorInsertmCh-GATA2-R396Q
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-mCh-Gata2
Plasmid#193061Purposedoxycycline-inducible expression of mCherry fused to mouse Gata2 in mammalian cellDepositorInsertmCh-Gata2
UseLentiviralTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-mCh-Gfi1b
Plasmid#193062Purposedoxycycline-inducible expression of mCherry fused to mouse Gfi1b in mammalian cellsDepositorInsertmCh-Gfi1b
UseLentiviralTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Gata2-mCh
Plasmid#193064Purposeconstitutive expression of mouse Gata2 fused to mCherry in mammalian cellsDepositorInsertGata2-mCh
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Gfi1b-mCh
Plasmid#193065Purposeconstitutive expression of mouse Gfi1b fused to mCherry in mammalian cellsDepositorInsertGfi1b-mCh
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD2529-CAG-b3 N305T
Plasmid#189798PurposeExpression of full length human integrin beta 3 with N305TDepositorInsertintegrin b3_N305T (ITGB3 Human)
UseTags-P2A-mCherryExpressionMammalianMutationN305TPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
Plasmid#184636PurposeExpresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGat1 (Slc6a1 Mouse)
UseAAVTagsExpressionMutationGat1 gene with Arginine 69 changed to Lysine, to …PromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gLacZ.EFS-NS.H2B-RFP
Plasmid#170363PurposeNegative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-KRAB-dCas9
Plasmid#120357PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [1-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7
Plasmid#118614Purposeexpression of mChe-tagged cdc42E7 under doxy-inducible CMV promoterDepositorInsertcdc42E7 (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationPromotertetON CMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRY-GalVP16 (B695)
Plasmid#92031PurposeExpresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGalBD-VP16ADExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
mGDF8 donor CFP-hDF
Plasmid#72590PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGDF8 donor CFP-hDF
Plasmid#72591PurposePlasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Fibrillarin-C1
Plasmid#134539PurposeExpresses Cherry-Fibrillarin fusion proteinDepositorInsertFibrillarin (FBL Human)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mitoGFP-DIO
Plasmid#174112PurposeCre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoterDepositorInsertmitoGFP (COX8A Human)
UseAAVTagsCox8 targeting sequence and GFPExpressionMutationPromoterEf1aAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only