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pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

Plasmid

#162815

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_M

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_M(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

Plasmid

#162816

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_9b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_9b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#115428

Purpose

Expresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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ACP2-V5

Plasmid

#113051

Purpose

Expresses worm ACP2 in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

ACid Phosphatase family 2 (acp-2 Nematode)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC84-ScSEIPIN-Ndelta

Plasmid

#96985

Purpose

Express yeast SEIPIN gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

ScSEIPIN (SEI1 Budding Yeast)

Use

Tags

6xHis and GFP

Expression

Plant

Mutation

N-terminal 39 nucleotides were deleted from yeast…

Promoter

35S

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TryX

Plasmid

#89623

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Tryparedoxin

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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GST-ZNF668

Plasmid

#45042

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 (ZNF668 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
PCx35CT-S276A, S298ter

Plasmid

#42914

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35 C-terminal domain

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

Ser276 to Ala and Ser298 stop codon

Promoter

T7

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S110A, S276A, S298ter

Plasmid

#42927

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Use

Tags

Expression

Mammalian

Mutation

Ser110 to Ala, Ser276 to Ala, and Ser298 to stop …

Promoter

CMV

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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KHBD00424

Plasmid

#34530

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6627 (Dnz1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pGSTag BimL(AA)

Plasmid

#25062

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (AA) (BCL2L11 Human)

Use

Tags

Gst

Expression

Bacterial

Mutation

Changed threonine 56 to alanine Changed serine 5…

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (S44D)

Plasmid

#24530

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(S44D) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (T56A)

Plasmid

#24247

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(T56A) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to alanine

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimL (S44A)

Plasmid

#24252

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL(S44A) (BCL2L11 Human)

Use

Tags

Gst

Expression

Bacterial

Mutation

Changed Serine 44 to Alanine

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimL (T56A)

Plasmid

#24253

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL(T56A) (BCL2L11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Changed threonine 56 to alanine

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimL (S58A)

Plasmid

#24254

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL(S58A) (BCL2L11 Human)

Use

Tags

Gst

Expression

Bacterial

Mutation

Changed serine 58 to alanine

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only