-
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No4
Plasmid#194899PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2DepositorInsertsgRNA targeting human MECP2 promoter No.4
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RFP-RGG
Plasmid#124944PurposeExoression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHis and mCherryExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_RAF1_94
Plasmid#111826PurposeKnockout Raf1DepositorInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
binder/tag-WT-FAK-insertionA
Plasmid#190439PurposeThe tag is SsrA (AANDENY). Tag is inserted after R35 of FAK.DepositorInsertFAK (Ptk2 Mouse)
UseTagsmCherryExpressionMammalianMutationSsrA inserted after aa 35PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYG026
Plasmid#65328Purposereporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.DepositorInsertccdB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH2829
Plasmid#199693PurposePcex-1 tomm-20::miniSOG UrSL mCherry unc-54 3' UTR C.elegans RIM neuron expression of miniSOG RFPDepositorInsertminiSOG
UseTagsRFPExpressionWormMutationPromoterPcex-1Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one_Bip gRNA1
Plasmid#183696PurposeExpresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selectiveDepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
Plasmid#176310PurposeExpresses CreN59-nMag-NLS with mCherry marker.DepositorInsertCreN59_nMag_NLS_P2A_mCherry_IRES_puroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No8
Plasmid#194902PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.8
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No6
Plasmid#194900PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.6
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No7
Plasmid#194901PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.7
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
eDrMet
Plasmid#183999PurposeOptically controlled eDrcMet expression in mammalian cellsDepositorInserteDrMet (MET Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFAP4_BHLH_3-2
Plasmid#185055PurposeDeletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2DepositorInsertTFAP4_3_2_gRNA (Tcfap4 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Plekhh1 5 silent mutations
Plasmid#187373PurposeExpresses human Plekhh1 with 5 silent mutations labelled with CherryDepositorInsertPlekhh1 with 5 silent mutations
UseTagsmCherryExpressionMammalianMutationFive silent mutations at the Plekhh1 siRNA site (…PromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS-KRAB-dCas9
Plasmid#120356PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-AncBE4max
Plasmid#174164PurposeThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.DepositorInsertAncBE4max
UseLentiviralTagsP2A-mCherryExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mouse b3 full length
Plasmid#217814PurposeFull length mouse integrin b3 expressionDepositorInsertITGB3 (Itgb3 Mouse)
UseTagsP2A-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only