Addgene

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pAc5.1B-EGFP-DmDCP1

Plasmid

#21684

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VAMP2 R125TAG

Plasmid

#69876

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2-GFP (Vamp2 Rat)

Use

Synthetic Biology

Tags

GFP

Expression

Mammalian

Mutation

Amber stop codon in the linker between VAMP2 and …

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-VTKS4-GFP

Plasmid

#178712

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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PKAcs-GGSD5X-GFP

Plasmid

#108584

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Use

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-ARF1-msGFP

Plasmid

#105255

Purpose

Plasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Arf1 (ARF1 Budding Yeast)

Use

Tags

msGFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pME-nls-sfGFP

Plasmid

#132973

Purpose

full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nuclear localized super folder GFP

Use

Gateway vector

Tags

6x myc and SV40 NLS

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-LynEGFP

Plasmid

#133923

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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YSBE 608 : pHYG_GFP

Plasmid

#92089

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-PA-GFP-His3MX6

Plasmid

#18822

Depositor

Wei-Lih Lee

Article

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Tags

Expression

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pDual-eGFP(Y203)

Plasmid

#63559

Purpose

Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

Depositor

Richard S Myers

Article

Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

Insert

His-T7-eGFP(Y203)

Use

Lentiviral

Tags

His6 and T7

Expression

Bacterial and Mammalian

Mutation

Changed threonine at position 203 with respect to…

Promoter

CMV-EF1α hybrid (CEF)

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pAG423GAL-EGFP-ccdB

Plasmid

#14317

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 S156A

Plasmid

#127588

Purpose

Phospho-mutant of S156

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Miro1 (RHOT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S156A

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-PIK1

Plasmid

#105260

Purpose

Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Pik1 (PIK1 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-TLG2

Plasmid

#105262

Purpose

Plasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg2 (TLG2 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43-YA/VD-GFP

Plasmid

#60703

Purpose

mammalian expression of rat Cx43 YA/VD mutant fused to GFP

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL14-GFP

Plasmid

#67406

Purpose

Mammalian expression of hArl14 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PsopB-GFPLVA

Plasmid

#23341

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

Plasmid

#196518

Purpose

Expression of Ctdnep1_D67E-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67EsiR (CTDNEP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead). Silent mutatio…

Promoter

CMV promoter; T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+CeGFP-Ctdnep1WTsiR

Plasmid

#196517

Purpose

Expression of Ctdnep1WT-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1WTsiR (CTDNEP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

CMV promoter; T7 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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