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Plasmid#119867PurposeDonor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-GFP-ATG101
Plasmid#171415PurposeFor the purifcation of GFP-ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN1735
Plasmid#127204PurposeDual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLSDepositorInsertpGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
1kb Zfp521 promoter reporter
Plasmid#104188Purposeluciferase reporter to assay Zfp521 promoter activationDepositorInsertZfp521 promoter - 1 Kb (Zfp521 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoternoneAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic
Plasmid#32734DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL4AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 deltaP
Plasmid#8596DepositorInsertSHP1 (PTPN6 Human)
UseTagsGSTExpressionBacterialMutationdeltaP (deletion of phosphatase domain, amino aci…PromoterAvailable sinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D_dC
Plasmid#176466Purposeexpress VPS13D lacking C-terminal 407 aa in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationC-terminal 407 aa is deleted.PromoterCMVAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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GST-GFP-ATG14
Plasmid#171426PurposeFor the purifcation of GFP- PI3KC3 complexDepositorInsertATG14 (ATG14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
M49 GST-TEV-Chibby-C
Plasmid#17197DepositorInsertChibby (CBY1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 64-126PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM1162
Plasmid#112535PurposepZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
UseTagsExpressionBacterialMutationPromoterJ23107 and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE
Plasmid#46593DepositorInsertmiR-16 Decoy (Mir16-1 Mouse, Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 DN
Plasmid#44831DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS25N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54
Plasmid#172451PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tagDepositorInsertTPD54 (TPD52L2 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_S176A
Plasmid#131202Purposemammalian expression, tet RDepositorInsertRIPK2 S176A (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationS176APromoterpCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC-OspCas12c-noncoding
Plasmid#120878PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-OspCas12
UseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-239
Plasmid#74609PurposeExpresses Hook domain and first coiled coil of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 CA
Plasmid#44825DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationQ67L (constitutive active)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only