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Plasmid#79877PurposeFLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianMutationPromoterCBhAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only
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pSHDY-Prha-mVenus_rhaS
Plasmid#137662PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.DepositorInsertPrha:mVenus
UseTagsExpressionBacterialMutationrhaS (Arg218Leu)PromoterPrhaAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTnos (GB0037)
Plasmid#68188PurposeProvides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid partDepositorInsertNos terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hAtg14
Plasmid#24295DepositorInsertautophagy related genes 14 (ATG14 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3E-mTagBFP
Plasmid#75175PurposeTagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertTagBFP
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3xFlag-ETO
Plasmid#12507DepositorInsertETO (RUNX1T1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMBPCas2/3
Plasmid#89238PurposePlasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusionDepositorInsertCas2/3
UseTagsStrep II-MBPExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
CH00X
Plasmid#73517PurposeTagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-TDepositorInsertpmex-5::TagRFP-T::PH::tbb-2
UseTagsTagRFP-TExpressionMutationPromotermes-5Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSplit2-NO-PRPF6
Plasmid#51740PurposeExpress LucN-PRPF6 fusion protein in mammalian cellsDepositorInsertPRP6 (PRPF6 Human)
UseTagsFLAG and Luciferase (1-398aa)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Nck1
Plasmid#45903DepositorInsertNck-1 (NCK1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorInsertNCK1 (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
YSBE 516 : pNEO_4xFLAG
Plasmid#92082PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRRH
Plasmid#136924PurposeExpresses Cas9-NRRH in mammalian cellsDepositorInsertCas9-NRRH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-miR-9 sponge
Plasmid#25040DepositorInsertmiR-9 sponge
UseRetroviralTagsd2EGFPExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG1280 NLS-mCherry-FIREtag-NES
Plasmid#205172Purposeexpresses NLS-mCherry-FIREtag-NES in mammalian cellsDepositorInsertNLS-mCherry-FIREtag-NES
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-emerin-EGFP-polyA
Plasmid#59747PurposeExpress emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertemerin-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 FLAG-SIV-RCM-vpx
Plasmid#115844PurposeEncodes codon optimized FLAG tagged SIVRCM-VpxDepositorInsertSIVRCM-Vpx
UseTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-hFAPP1 PH domain
Plasmid#58723PurposeExpresses GFP-Tagged human FAPP1 PH domain in yeastDepositorInsertFour-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
YSBE 517 : pNEO_6xHA
Plasmid#92083PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only