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Plasmid#50474PurposeGq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only
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pCAGGS-flpE-puro
Plasmid#20733DepositorInsertFlpE recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSpCTRE-CD4
Plasmid#114010PurposeDox-inducible Cas9 expression with a CD4 selection markerDepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScarlet-N1
Plasmid#128060PurposeMammalian expression vector for C terminal mScarlet-I fusionsDepositorTypeEmpty backboneUseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-10A1
Plasmid#15805DepositorInsert10A1 Murine leukemia virus envelope mRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAc-C Med12His
Plasmid#49240Purposeexpresses human Med12 with His tag in insect cellsDepositorInsertMed12 (MED12 Human)
UseTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRV.GFP FOXP3
Plasmid#13250DepositorInsertFOXP3 (FOXP3 Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
a1Ha-pcDNA3
Plasmid#45809DepositorInserta1Ha (CACNA1H Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG
Plasmid#85225PurposeExpresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsUseAAV and Cre/LoxTagsExpressionMutationPromoterhSynAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
anxA2-GFP
Plasmid#107196PurposeExpresses human annexin A2-GFP fusion protein for live cell imagingDepositorInsertannexin A2 (ANXA2 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationAla residue 65 in human sequence replaced by Glu ā¦PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D1R
Plasmid#89160PurposeExpress vaccinia virus D1R in mammalian cellsDepositorInsertVaccinia virus D1R
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XMyc-EGFP-PEX26
Plasmid#139059PurposeMyc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XMyc-EGFP-PEX26
UseLentiviralTagsMycExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF2-ABCG2-IRES-Neo
Plasmid#25983DepositorInsertATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
UseLentiviralTagsEF1-apha promoter and IRES-NeoExpressionMammalianMutationNonePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
PARP1 FL
Plasmid#169815PurposeExpresses codon optimised full length His tagged PARP1 in bacterial cellsDepositorInsertPoly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
UseTagsMKHHHHHHMKQExpressionBacterialMutationValine 762 mutated to an alaninePromoterT7 promoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-BioID-FAM21
Plasmid#121046PurposeExpresses GFP tagged BioID and human FAM21C in mammalian cellsDepositorInsertsUseTagsGFPExpressionMammalianMutationR118G (highly promiscuous form)PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-ETV2-IRES-Puro
Plasmid#61061Purposelentiviral vector for ETV2DepositorInsertETV2 (ETV2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/MG1.1
Plasmid#184540PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsidDepositorInsertAAV-MG1.1 capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R, A587L, Q588M; inseā¦PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sgAAVS1
Plasmid#192480PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as controlDepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_AOX-FLAG
Plasmid#177984PurposeAll-in-one dox-inducible expression of Alternative OxidaseDepositorInsertAOX
UseLentiviralTagsFLAGExpressionMammalianMutationCodon optimized cDNA for expression in human cellsPromoterTREAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only