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Plasmid#195137PurposegRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Downstream gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_CRISPRi_gRNA_2
Plasmid#195133PurposegRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherryDepositorInsertMDM4 CRISPRi gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_CRISPRi_gRNA_1
Plasmid#195132PurposegRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherryDepositorInsertMDM4 CRISPRi gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Downstream_gRNA_1
Plasmid#195136PurposegRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Downstream gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1ORF43(1-41)-3xFLAG
Plasmid#130881PurposeExpresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tagDepositorInsertC1ORF43 (C1orf43 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationDeleted amino acids 44-253PromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2462
Plasmid#97064PurposeExpressing Sulfolobus solfataricus orf SSO2462 proteinDepositorInsertS. solfataricus orf SSO2462
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2452
Plasmid#97059PurposeExpressing Sulfolobus solfataricus orf SSO2452 proteinDepositorInsertS. solfataricus orf SSO2452
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF126
Plasmid#97021PurposeExpressing Pyrobaculum spherical virus orf126 proteinDepositorInsertPSV orf 126
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA5006
Plasmid#97016PurposeExpressing Pseudomonas aeruginosa orf PA5006 proteinDepositorInsertP aeruginosa orf PA5006
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2-FLL[157-159]AAA
Plasmid#96994PurposeExpress GFP-tagged mutated mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationAmino acid residues 157-159 were mutated (FLL[157…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2-FLL[157-159]AAA
Plasmid#96991PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationAmino acid residues 157-159 were mutated (FLL[157…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA0856
Plasmid#97014PurposeExpressing Pseudomonas aeruginosa orf PA0856 proteinDepositorInsertP aeruginosa orf PA0856
UseTagsN-ter TEV cleavable 6HIsExpressionMutationN-terminal deletion (AA 1-34)PromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4631
Plasmid#97017PurposeExpressing Pseudomonas aeruginosa orf PA4631 proteinDepositorInsertNucleoside-diphosphate-sugar epimerase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2373
Plasmid#97058PurposeExpressing Sulfolobus solfataricus orf SSO2373 proteinDepositorInsertS. solfataricus orf SSO2373
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHISSSO1986
Plasmid#97057PurposeExpressing Sulfolobus solfataricus orf SSO1986 proteinDepositorInsertS. solfataricus orf SSO1986
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPApqsL
Plasmid#97020PurposeExpressing Pseudomonas aeruginosa pqsL proteinDepositorInsertPutative FAD-dependent monooxygenase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSAPPFK
Plasmid#97003PurposeExpressing MRSA PPFK proteinDepositorInsertPutative phosphofructokinase
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSO1404
Plasmid#97056PurposeExpressing Sulfolobus solfataricus orf SSO1404 proteinDepositorInsertS. solfataricus orf SSO1404
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF239
Plasmid#97025PurposeExpressing Pyrobaculum spherical virus orf239 proteinDepositorInsertPSV orf 239
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF165A
Plasmid#97024PurposeExpressing Pyrobaculum spherical virus orf165a proteinDepositorInsertPSV orf 165a
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only