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Plasmid#19480DepositorInsertHSPH1 (HSPH1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NP-B/BRISBANE/60/2008
Plasmid#187921PurposeExpresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cellsDepositorInsertInfluenza B Nucleoprotein
UseTagsUNTAGGEDExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Golgi-ABKAR
Plasmid#61507PurposeExpression of AMPK biosensor targeted to the GolgiDepositorInsertAMPK Biosensor
UseTagsGolgi-targeting signal (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nme2sgRNA_pLKO
Plasmid#119926PurposeNme2Cas9 U6-driven sgRNA cassetteDepositorInsertNmeCas9 sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-ABKAR
Plasmid#65068PurposeExpression of ABKAR biosensor targeted to the lysozomeDepositorInsertABKAR biosensor
UseTagsLamp1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zeb2-3'UTR
Plasmid#61771PurposeLuciferase reporter containing the 3'UTR of mouse Zeb2DepositorInsertmouse Zeb2 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB8
Plasmid#19504DepositorInsertDNAJB8 (DNAJB8 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNLS (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG
Plasmid#79877PurposeFLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianMutationPromoterCBhAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTnos (GB0037)
Plasmid#68188PurposeProvides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid partDepositorInsertNos terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-Prha-mVenus_rhaS
Plasmid#137662PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.DepositorInsertPrha:mVenus
UseTagsExpressionBacterialMutationrhaS (Arg218Leu)PromoterPrhaAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CH00X
Plasmid#73517PurposeTagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-TDepositorInsertpmex-5::TagRFP-T::PH::tbb-2
UseTagsTagRFP-TExpressionMutationPromotermes-5Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBPCas2/3
Plasmid#89238PurposePlasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusionDepositorInsertCas2/3
UseTagsStrep II-MBPExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hAtg14
Plasmid#24295DepositorInsertautophagy related genes 14 (ATG14 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorInsertNCK1 (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-mTagBFP
Plasmid#75175PurposeTagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertTagBFP
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3xFlag-ETO
Plasmid#12507DepositorInsertETO (RUNX1T1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSplit2-NO-PRPF6
Plasmid#51740PurposeExpress LucN-PRPF6 fusion protein in mammalian cellsDepositorInsertPRP6 (PRPF6 Human)
UseTagsFLAG and Luciferase (1-398aa)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Nck1
Plasmid#45903DepositorInsertNck-1 (NCK1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
YSBE 516 : pNEO_4xFLAG
Plasmid#92082PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only