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Addgene
Showing: 5661 - 5680 of 16015 results
  1. pFA6a-SRM (sortase recognition motif)-hphMX4

    Plasmid
    #54488
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    hphMX4
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pEYFP-C1-NuMA

    Plasmid
    #28238
    Purpose
    Expresses human NuMA with N-terminal EYFP tag in mammalian cells
    Depositor
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pEGFP-C1-NuMA

    Plasmid
    #81029
    Purpose
    Expresses human NuMA with N-terminal EGFP tag in mammalian cells
    Depositor
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. TBRII-N-18

    Plasmid
    #58196
    Purpose
    Localization: Membrane Receptor, Excitation: N/A, Emission: N/A
    Depositor
    Insert
    TBRII (TGFBR2 Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pCer-C3-Plk1-T210D

    Plasmid
    #68133
    Purpose
    Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells
    Depositor
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    T210D constitutively active version
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pCer-C3-Plk1-K82R

    Plasmid
    #68134
    Purpose
    Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells
    Depositor
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    K82R kinase-dead version
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  7. Clathrin-15

    Plasmid
    #58190
    Purpose
    Localization: Clathrin Vesicles, Excitation: N/A, Emission: N/A
    Depositor
    Insert
    Clathrin (CLTB Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pDS-Red-N1 GABAA beta3

    Plasmid
    #109143
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.
    Depositor
    Insert
    GABAa receptor
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    beta3 subunit of the GABAa receptor
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  9. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

    Plasmid
    #72608
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation
    Depositor
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine37 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

    Plasmid
    #72609
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation
    Depositor
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed threonine34 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

    Plasmid
    #72610
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation
    Depositor
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine33 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 5661 - 5680 of 16015 results