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Plasmid#27496DepositorInsertBRCC36 (BRCC3 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-GFP-Tdtomato-iRFP670
Plasmid#111812PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
Tdtomato
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mCherry
Plasmid#166024PurposeExpresses human PFAS-mCherry fusion in mammalian cellsDepositorInsertPFAS-mCherry (PFAS Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(WT)
Plasmid#84639PurposemCherry-tagged, wild-type LKB1DepositorInsertmCherry-LBK1 (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10
Plasmid#127129DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK24R- please see depositor commentsPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag WDR5
Plasmid#15552DepositorInsertWdr5 (Wdr5 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)1
Plasmid#127089PurposeBacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP human SUMO3 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-Nla-repeat-BbsI
Plasmid#180215PurposeFor cloning Neisseria lactamica Type I-C crRNA for protein purification in E.ColiDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PAK2 binding domain
Plasmid#83951PurposeFLIM acceptor for mEGFP Rac1DepositorInsertPak2 (PAK2 Human)
UseTagsmCherryExpressionMammalianMutationaa60-113 only; S74A F84APromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-eYFP
Plasmid#104056PurposeAn AAV genome with tet-inducible expression of the fluorescent protein eYFPDepositorInsertEYFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2
Plasmid#27415DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTags2xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT tetramutant for Split-PE (pEH101)
Plasmid#190109PurposeExpresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationD14R, D74R, N116K, N197RPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPH1
Plasmid#19480DepositorInsertHSPH1 (HSPH1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Nme2sgRNA_pLKO
Plasmid#119926PurposeNme2Cas9 U6-driven sgRNA cassetteDepositorInsertNmeCas9 sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Golgi-ABKAR
Plasmid#61507PurposeExpression of AMPK biosensor targeted to the GolgiDepositorInsertAMPK Biosensor
UseTagsGolgi-targeting signal (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-ABKAR
Plasmid#65068PurposeExpression of ABKAR biosensor targeted to the lysozomeDepositorInsertABKAR biosensor
UseTagsLamp1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB8
Plasmid#19504DepositorInsertDNAJB8 (DNAJB8 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zeb2-3'UTR
Plasmid#61771PurposeLuciferase reporter containing the 3'UTR of mouse Zeb2DepositorInsertmouse Zeb2 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNLS (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only