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pCRRNA-G20-R0

Plasmid

#101807

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(935-984)

Plasmid

#42029

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa935-984

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(1027-1076)

Plasmid

#42019

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1027-1076

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(935-1026)

Plasmid

#42018

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa935-1026

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(925-934)

Plasmid

#42017

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa925-934

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(1077-1709)

Plasmid

#42016

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1077-1709

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4NT

Plasmid

#62261

Purpose

expression of A4NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #409 (Sa)

Plasmid

#98134

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #409

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #351 (Sa)

Plasmid

#105283

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #351

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_SOX5_1

Plasmid

#86357

Purpose

Encodes gRNA for 3' target of human SOX5

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SOX5 (SOX5 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pADH143-2

Plasmid

#90990

Purpose

NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pADH100-2

Plasmid

#90981

Purpose

NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_4

Plasmid

#73528

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-A3T

Plasmid

#62260

Purpose

expression of A3T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSuper-TatSF1 AB

Plasmid

#31119

Depositor

Mariano Garcia-Blanco

Article

Miller et al PLoS One. 2009 . 4(5):e5710.

Insert

TatSF1 shRNA (HTATSF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pmyc-GFP-TNRC6A-del.GW-I+II+III

Plasmid

#42033

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532, aa716-772 and aa804-924

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G13 mutant

Plasmid

#231915

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G13 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G14 mutant

Plasmid

#231916

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G14 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS-CoV-2 spike G15 mutant

Plasmid

#231917

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G15 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G145 mutant

Plasmid

#231918

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G145 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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