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Plasmid#97159PurposeFor dual luciferase report assayDepositorInsertDre-Rac2 3'UTR
UseTagsExpressionMammalianMutationmiR-722 Binding MutantPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-zRac2 3'UTR
Plasmid#97158PurposeFor dual luciferase report assayDepositorInsertDre-Rac2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC S431A T432A NS1
Plasmid#101837Purposedrives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locusDepositorInsertkaiBC S431A T432A with p0050 promoter
UseCyanobacteria cloning vectorTagsExpressionMutationS431A T432APromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC S431E T432E NS1
Plasmid#101836Purposedrives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locusDepositorInsertkaiBC S431E T432E with p0050 promoter
UseCyanobacteria cloning vectorTagsExpressionMutationS431E T432EPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-ProUBQ10 GW
Plasmid#79751Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterProUBQ10Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LGhG-pHG165a
Plasmid#90048PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLGhG
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-XbaI
Plasmid#87328PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-XhoI
Plasmid#87330PurposeTo express shRNA against human Nup93DepositorInsertshRNA against human Nup93
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB172
Plasmid#86992PurposeCRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1DepositorInsertgRNA targeting pil1
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB(KTF)-HA no-pA
Plasmid#82596Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationW2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW022
Plasmid#83532Purposepheromone mediated expression of UBiC, ARO4, and TKL1DepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW015
Plasmid#83530PurposeUBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoterDepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHBA03
Plasmid#83537PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genesDepositorInsertpTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB6
Plasmid#83557PurposeUsed to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.DepositorInsertGAL10 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)
Plasmid#84661Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailable sinceNov. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)
Plasmid#84662Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailable sinceNov. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2B
Plasmid#82373PurposedCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas45
Plasmid#82390PurposesgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas7
Plasmid#82379PurposedCas9 expressed from low constituve promoter, c225, in acsADepositorInsertdCas9
UseTagsExpressionMutationPromoterc225Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2E
Plasmid#82376PurposedCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only