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pegRNA-GFP>BFP_Y66H-NGG

Plasmid

#185480

Purpose

pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP>BFP_Y66H-pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGM234

Plasmid

#220177

Purpose

Expresses exogenous wild-type NAC complex members (NACA and BTF3)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

NACA and BTF3 (NACA, BTF3 Human)

Use

Lentiviral

Tags

3xFLAG (NACA); 6xHis (BTF3)

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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StrKDEL-IRES-mannosidase II-mTagBFP2

Plasmid

#165460

Purpose

Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.

Depositor

David Stephens

Article

McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

Insert

Partial ManII-mTag2BFP fusion (Man2a1 Mouse)

Use

Tags

Str-KDEL and mTag2BFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_2

Plasmid

#195131

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 2 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_1

Plasmid

#195130

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 1 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTrcHisB mLCAD (29-430 aa)

Plasmid

#24490

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-28 deleted encodes active 29-430

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1mLCAD_8KR_FLAG

Plasmid

#24485

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pMDC43-FIT2-N[80]A

Plasmid

#96995

Purpose

Express GFP-tagged mutated mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Use

Tags

GFP

Expression

Plant

Mutation

Amino acid residue 80 was mutated (N[80]A). Mutat…

Promoter

35S

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEHISSSO6206

Plasmid

#97065

Purpose

Expressing Sulfolobus solfataricus orf SSO6206 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO6206

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3

Plasmid

#136364

Purpose

Expresses epitope-tagged human TET3 WT in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-MDM4

Plasmid

#195140

Purpose

Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 (MDM4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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C1ORF43-3xFLAG

Plasmid

#130880

Purpose

Expresses human C1ORF43 with C-terminal 3xFLAG tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) eGFP

Plasmid

#107505

Purpose

Expresses eGFP, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CASANOVA

Plasmid

#113036

Purpose

AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) FITM2

Plasmid

#107504

Purpose

Expresses FITM2, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FITM2 (codon optimized)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsE

Plasmid

#97019

Purpose

Expressing Pseudomonas aeruginosa pqsE protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Quinolone signal response protein

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2676

Plasmid

#96970

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 2676

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Sar2676

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAMVAK

Plasmid

#97000

Purpose

expression of methicillin-resistant Staphylococcus aureus MVAK protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Mevalonate kinase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPAFabH

Plasmid

#97018

Purpose

Expressing Pseudomonas aeruginosa FabH protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

3-Oxoacyl-[acyl-carrier-protein] synthase III

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGM238

Plasmid

#220178

Purpose

Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

NACA and BTF3 (NACA, BTF3 Human)

Use

Lentiviral

Tags

3xFLAG (NACA); 6xHis (BTF3)

Expression

Mutation

K78E (NACA) + K43B (BTF3)

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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