Addgene

We narrowed to 6,255 results for: sms

Showing: 5641 - 5660 of 6255 results

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pCASCADE-gltA2-xylA

Plasmid

#158613

Purpose

Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

gltA2-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-zwf-xylA

Plasmid

#158614

Purpose

Low phosphate inducible gRNA array to silence the xylA and zwf promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

zwf-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF3∆165N (H348E)

Plasmid

#175420

Purpose

Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Use

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N, H348E

Promoter

T7

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh-1478

Plasmid

#82424

Purpose

Plasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-MAP4-C-10

Plasmid

#57794

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

MAP4

Use

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
miniSOG-MAP4-C-10

Plasmid

#57772

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

MAP4

Use

Tags

miniSOG

Expression

Mammalian

Mutation

G982V and G993C compared to NM_008633.4.

Promoter

CMV

Available since

Dec. 7, 2015

Availability

Industry, Academic Institutions, and Nonprofits
NET33 pEGFP-N2 (594)

Plasmid

#61987

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-CAF1-C-10

Plasmid

#57784

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

CAF1

Use

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-C-Src-7

Plasmid

#57785

Purpose

Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

C-Src

Use

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
miniSOG-VASP-5

Plasmid

#57778

Purpose

Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

VASP

Use

Tags

miniSOG

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
miniSOG-Talin-C-10

Plasmid

#57776

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Talin

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdminiSOG-RatLC3

Plasmid

#57807

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Use

Tags

tdminiSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-miniSOG-Talin-N-10

Plasmid

#55096

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Talin

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Profilin-10

Plasmid

#55093

Purpose

Localization: Cytoskeleton, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Profilin

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa

Plasmid

#127389

Purpose

Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Tagger

Plasmid

#129396

Purpose

Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

Insert

rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2

Use

Tags

FLAG, HA, and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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