Addgene

We narrowed to 51,918 results for: cloning

Showing: 5641 - 5660 of 51918 results

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pQTEV-NEUGRIN

Plasmid

#34804

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

NEUGRIN (NGRN Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pICH87644

Plasmid

#50274

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1479

Plasmid

#50291

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM5331

Plasmid

#50294

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1482

Plasmid

#50295

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + mitochondrial localisation signal ScCoxIV (Saccharomyces cerevisiae)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45195

Plasmid

#50275

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH85281

Plasmid

#50272

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-C10orf83

Plasmid

#34827

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

C10orf83 (MORN4 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM5343

Plasmid

#50297

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB7

Plasmid

#19557

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pTOPO-rRNA-5‘ETS 347 to 631

Plasmid

#23994

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rRNA 5'ETS ntds 347 to 631

Use

Bacterial cloning - topo

Tags

Expression

Mutation

None

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL452

Plasmid

#219502

Purpose

Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1333-DddA11'-C

Plasmid

#199797

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1333-DddA11'-C

Plasmid

#199798

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NI_G1333-DddA11'-C

Plasmid

#199799

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NN_G1333-DddA11'-C

Plasmid

#199800

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_HD_G1333-DddA11'-N

Plasmid

#199801

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NG_G1333-DddA11'-N

Plasmid

#199802

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge