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Plasmid#194018PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionMutationPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTarget-Dual
Plasmid#207884PurposeCarries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.DepositorInsertsI-OnuI target site
I-GpeMI target site
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspB
Plasmid#215400PurposeCm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)DepositorInsertcspB
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA2
Plasmid#215399PurposeCm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)DepositorInsertcspA2
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN667
Plasmid#219963PurposeExpressed sfGFP fused human KIF1Bß(1-721) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationencodes 1-721PromoterpolyhedrinAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN668
Plasmid#219962PurposeExpressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-EEA1
Plasmid#222419PurposeTo express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertEEA1 (EEA1 Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-BAF
Plasmid#222414PurposeTo express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertBAF (BANF1 Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Talin1 head EGFP fusion
Plasmid#221408PurposeMammalian expression of Talin1 head EGFP fusion for mammalian expressionDepositorInsertTalin1 head EGFP fusion (TLN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-5
Plasmid#220442PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)DepositorInsertPUF3 (puf-3 Nematode)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA
Plasmid#207064PurposeExpressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.DepositorInsertPex3
UseTags4xV5-APEX2 and GFPExpressionYeastMutationPromoterpADH3Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00029 (aka pRC0610)
Plasmid#200834PurposeLentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-ERM
Plasmid#217377PurposeER markerDepositorInsertrat cb5 ERM
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS59
Plasmid#215680PurposeCas9 + guide plasmid targeting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS77
Plasmid#215681PurposeCas9 + guide plasmid targeting ChrIII split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Snf7-4V5-APEX2-URA
Plasmid#207065PurposeModerate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.DepositorInsertSnf7
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…PromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Vd-ARATR-GFP
Plasmid#208903Purposeencodes the arachnotocin receptor (oxytocin/vasopressin-like) from the mite Varroa destructor, tagged with GFPDepositorInsertARATR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only